Creation of cell models from confocal microscopy data files using rapid prototyping

Abstract

A novel process was developed to convert digital data files from confocal microscopy of cells into three‐dimensional models. This process produces accurate models of cells as compared to existing methods that primarily rely on renditions. Data sets form Confocal Laser Scanning Microscopy (CLSM) and Multiphoton Laser Scanning Microscopy (MPLSM) for C. elegans embryo were converted to TIFF files using open source program Image J. Following importing files in Mimics, a medical imaging program, a slice distance of 4uM was chosen for modeling. Following enhancement, a Boolean operation was performed creating 2 separate segmentation masks, one for cellular structures and the other for exterior wall of embryo. The files were then exported into Freeform Modeling program which allowed us to convert 3‐D computer images into virtual clay. Files were further converted to a .stl format which can be read by rapid prototyping machine spectrum ZTM 510 (Z Corporation) 3D printer. Another build using 3D systems 250 SLA employing the DSM Somos Watershed XC 11122 resin was accomplished. The novel process described for building accurate 3‐D models of cells could find use in producing specimens for educational, research and clinical applications. Supported by National Science Foundation, grant EEC‐0648845 awarded to Subha Kumpaty, Department of Mechanical Engineering, Milwaukee School of Engineering.