Cortical structure of macroalgae influences epiphytic eukaryotic assemblages

BackgroundLoss of cortical volume in frontotemporal regions has been reported in patients with schizophrenia and their relatives. Cortical area and thickness are determined by different genetic processes, and measuring these parameters separately may clarify disturbances in corticogenesis relevant to schizophrenia. Our study also explored clinical and cognitive correlates of these parameters.MethodsThirty-seven patients with first-episode psychosis (34 schizophrenia, 3 schizoaffective disorder) and 38 healthy control subjects matched for age and sex took part in the study. Imaging was performed on an magnetic resonance imaging 1.5-T scanner. Area and thickness of the frontotemporal cortex were measured using a surface-based morphometry method (Freesurfer). All subjects underwent neuropsychologic testing that included measures of premorbid and current IQ, working and verbal memory, and executive function.ResultsReductions in cortical area, more marked in the temporal cortex, were present in patients. Overall frontotemporal cortical thickness did not differ between groups, although regional thinning of the right superior temporal region was observed in patients. There was a significant association of both premorbid IQ and IQ at disease onset with area, but not thickness, of the frontotemporal cortex, and working memory span was associated with area of the frontal cortex. These associations remained significant when only patients with schizophrenia were considered.ConclusionsOur results suggest an early disruption of corticogenesis in schizophrenia, although the effect of subsequent environmental factors cannot be excluded. In addition, cortical abnormalities are subject to regional variations and differ from those present in neurodegenerative diseases.

1. Methods that assess patterns of phylogenetic relatedness, as well as character distribution and evolution, allow one to infer the ecological processes involved in community assembly. Assuming niche conservatism, assemblages should shift from phylogenetic clustering to evenness with decreasing geographic scale because the relative importance of mechanisms that shape assemblages is hypothesized to be scale-dependent. Whereas habitat filtering is more likely to act at regional scales because of increased habitat heterogeneity that allows sorting of ecologically similar species in contrasting environments, competition is more likely to act at local scales because low habitat heterogeneity provides few opportunities for niche partitioning. 2. We used species lists to assess assemblage composition, data on ecologically-relevant traits, and a molecular phylogeny, to examine the phylogenetic structure of antbird (Thamnophilidae) assemblages at three different geographical scales: regional (ecoregions), intermediate (100-ha plots) and local (mixed-flocks). In addition, we used patterns of phylogenetic beta diversity and beta diversity to separate the factors that structure antbird assemblages at regional scales. 3. Contrary to previous findings, we found a shift from phylogenetic evenness to clustering with decreasing geographical scale. We argue that this does not reject the hypothesis that habitat filtering is the predominant force in regional community assembly, because analyses of trait evolution and structure indicated a lack of niche conservatism in antbirds. 4. In some cases, phylogenetic evenness at regional scales can be an effect of historical biogeographic processes instead of niche-based processes. However, regional patterns of beta diversity and phylogenetic beta diversity suggested that phylogenetic structure in our study cannot be explained by the history of speciation and dispersal of antbirds, further supporting the habitat-filtering hypothesis. 5. Our analyses suggested that competitive interactions might not play an important role locally, which would provide a plausible explanation for the high alpha diversity of antbirds in Amazonia. 6. Finally, we emphasize the importance of including trait information in studies of phylogenetic community structure to adequately assess the mechanisms that determine species co-existence.

Root diameter, a critical indicator of root physiological function, varies greatly among tree species, but the underlying mechanism of this high variability is unclear. Here, we sampled 50 tree species across tropical and temperate zones in China, and measured root morphological and anatomical traits along the first five branch orders in each species. Our objectives were (i) to reveal the relationships between root diameter, cortical thickness and stele diameter among tree species in tropical and temperate forests, and (ii) to investigate the relationship of both root morphological and anatomical traits with divergence time during species radiation. The results showed that root diameter was strongly affected by cortical thickness but less by stele diameter in both tropical and temperate species. Changes in cortical thickness explained over 90% of variation in root diameter for the first order, and ∼74-87% for the second and third orders. Thicker roots displayed greater cortical thickness and more cortical cell layers than thinner roots. Phylogenetic analysis demonstrated that root diameter, cortical thickness and number of cortical cell layers significantly correlated with divergence time at the family level, showing similar variation trends in geological time. The results also suggested that trees tend to decrease their root cortical thickness rather than stele diameter during species radiation. The close linkage of variations in root morphology and anatomy to phylogeny as demonstrated by the data from the 50 tree species should provide some insights into the mechanism of root diameter variability among tree species.

Background and purpose: Previous voxel-based morphometry (VBM) studies have suggested that cortical atrophy is regionally distributed in middle-aged subjects with white matter hyperintense (WMH) lesions. However, few studies have assessed cortical thickness in middle-aged WMH subjects. In this study, we examined cortical thickness as well as cortical morphometry associated with the presence of WMH lesion load in middle-aged subjects.Participants and methods: Thirty-six middle-aged subjects with WMH lesions (WMH group) and without clinical cognitive impairment, and 34 demographically matched healthy control subjects (HCS group) participated in the study. Cortical thickness was estimated using an automated Computational Anatomy Toolbox (CAT12) as the distance between the gray-white matter border and the pial surface. Individual WMH lesions were manually segmented, and WMH loads were measured. Statistical cortical maps were created to estimate differences in cortical thickness between groups based on this cortex-wide analysis. The relationship between WMH lesion loads and cerebral cortical thickness was also analyzed in CAT12.Results: Cortical thickness was significantly lower in the WMH group than in the controls in multimodal integration regions, including the right and left dorsal anterior cingulate cortex (dACC), right and left frontal operculum (fO), right and left operculum parietale (OP), right and left middle temporal gyrus (MTG), and left superior temporal gyrus (STG; P < 0.01, family-wise error (FWE)-corrected). Additionally, cortical thickness was also lower in the recognition regions that contained the right temporal pole (TP), the right and left fusiform gyrus, and the left rolandic operculum (RO; P < 0.01, FWE-corrected). The results revealed that in the left superior parietal lobule (SPL), cortical thickness was higher in the WMH group than in the HCS group (P < 0.01, FWE-corrected). A voxel-wise negative correlation was found between cortical thickness and WMH lesion loads in the right orbitofrontal cortex (OFC), right dorsolateral prefrontal cortex (DLPFC), and right subcallosal cortex (P < 0.01, FWE-corrected).Conclusion: The main findings of this study suggest that middle-aged WMH subjects are more likely to exhibit cortical thinning, especially in multimodal integration and recognition- and motor-related regions. The current morphometry data provide further evidence for WMH-associated structural plasticity.

The purpose of this study was to determine the accuracy of Cone Beam Computerized Tomography (CBCT) reconstructions in displaying bone surface size and cortical layer thickness. Two fresh frozen cadaver heads were scanned using a CBCT (i-CAT(™) 3D Imaging System; Imaging Sciences International Inc.). The mandibles were sectioned and digitalized for histological evaluation. Dimensions as measured on these sections were compared with CBCT measurements of the same region with the use of 3D image-based planning software (Procera System NobelGuide(™); Nobel Biocare). To allow optimal comparison between histological and CBCT sections, reference markers were placed into the mandibles. The Student t-test was utilized to analyze the data. Differences with P-values <0.05 were considered significant. The total height and width of the mandibular body, as measured on CBCT views, were larger compared with the histological values with a maximum difference of 0.33 mm (SD ± 0.34). Cortical thickness measurements were significantly thicker on CBCT sections (P < 0.006) with absolute differences ranging up to ±0.63 mm (SD ±0.28) and relative differences ranging up to 82.6%. CBCT images (i-CAT 3D Imaging System), viewed on the planning software Procera System NobelGuide, tend to overestimate the anatomical truth in assessing both bone height and width; especially cortical thicknesses are depicted thicker than in reality. When using CBCT images for presurgical assessment, one should be aware of this phenomenon of exaggeration of dimensions.

The absolute number of neurons and thickness of the cortex were studied in areas 6, 10, 18, 21, 21/38 and 40 and in the subiculum of mentally healthy persons of different ages and in patients with senile and vascular dementia or with Pick's or Alzheimer's disease. In old age the mean absolute number of neurons in mentally healthy persons is reduced by 20%, while the thickness of the cortex on the free surface of the gyri remains unchanged. In persons with senile and vascular dementia the number of nerve cells is reduced by 35–38%, but there is no decrease in the thickness of the cortex on its free surface. In Alzheimer's disease the number of nerve cells is reduced by half and the thickness of the cortex by 6%. Pick's disease is characterized by mass death of nerve cells in the affected areas, leading to a reduction of 14–30 in their number, and to a decrease in thickness of the cortex by half. The subicular cortex thickness in old age is reduced by 28% and in diseases leading to dementia by 47–71%.

BackgroundThe geographic patterns of plant diversity in the Qinghai-Tibet Plateau (QTP) have been widely studied, but few studies have focused on wetland plants. This study quantified the geographic patterns of wetland plant diversity in the QTP through a comprehensive analysis of taxonomic, phylogenetic and functional indices.MethodsBased on a large number of floras, monographs, specimens and field survey data, we constructed a comprehensive dataset of 1,958 wetland plant species in the QTP. Species richness (SR), phylogenetic diversity (PD), functional diversity (FD), net relatedness index (NRI) and net functional relatedness index (NFRI) were used to assess the taxonomic, phylogenetic and functional diversity of wetland plants. We explored the relationships between the diversity indices and four categories of environmental variables (i.e. energy-water, climate seasonality, topography and human activities). We used four diversity indices, namely endemic species richness, weighted endemism, phylogenetic endemism and functional endemism, together with the categorical analysis of neo- and paleo-endemism (CANAPE), to identify the endemic centers of wetland plants in the QTP.ResultsSR, PD and FD were highly consistent and showed a decreasing trend from southeast to northwest, decreasing with increasing elevation. The phylogenetic structure of wetland plant assemblages in most parts of the plateau is mainly clustered. The functional structure of wetland plant assemblages in the southeast of the plateau is overdispersed, while the functional structure of wetland plant assemblages in other areas is clustered. Energy-water and climate seasonality were the two most important categories of variables affecting wetland plant diversity. Environmental variables had a greater effect on the functional structure of wetland plants than on the phylogenetic structure. This study identified seven endemic centres, mainly in the Himalayas and Hengduan Mountains.ConclusionsClimate and topography are the main factors determining the geographic distribution of wetland plant diversity at large scales. The majority of grid cells in the QTP with significant phylogenetic endemism were mixed and super-endemism. At large scales, compared to climate and topography, human activities may not have a negative impact on wetland plant diversity in the QTP.

What humeri are suitable for comparative testing of suture anchors? An ultrastructural bone analysis and biomechanical study of ovine, bovine and human humeri and four different anchor types

In osteoporosis, total bone mass decreases and the thickness of the cortical layer diminishes in the shafts of the long bones. In this study, a simple ultrasonic in vivo method for determining the thickness of the cortical bone layer was applied, and the suitability of two different signal analysis techniques, i.e., envelope and cepstral methods, for measuring cortical thickness was compared. The values of cortical thickness, as determined with both techniques, showed high linear correlations (r > or = 0.95) with the thickness values obtained from in vitro measurements with a caliper or in vivo measurements by peripheral quantitative CT (pQCT). No systematic errors that could be related to the cortical thickness were found. The in vivo accuracy of the measurements was 6.6% and 7.0% for the envelope and cepstral methods, respectively. Further, the in vivo precision for the envelope and cepstral methods was 0.26 mm and 0.28 mm, respectively. Although the results are similar for both of the techniques, the simplicity of the envelope method makes it more attractive for clinical applications. In conclusion, a simple ultrasound measurement provides an accurate estimate of the cortical bone thickness. The techniques investigated may have clinical potential for osteoporosis screening and therefore warrant more extensive clinical investigations with healthy and osteoporotic individuals.

Morphological alterations in the congenital blind based on the analysis of cortical thickness and surface area

BackgroundUnderstorey vegetation is a key biodiversity component of forest ecosystems. Previous studies examined its relations with the overstorey mainly in terms of taxonomic diversity, composition or productivity. So far, none focused on the phylogenetic aspect, which represents the deepest component of diversity in a community. Here, we explored the relations between overstorey species richness and identity and the phylogenetic structure of the understorey vegetation. As a model system we used a network of 36 plots in thermophilous deciduous forests of central Italy that are part of a European project on forest biodiversity and functions. The plots, characterized by similar site conditions, represent a gradient of overstorey richness from 1 to 4-species mixtures, with variable composition. After surveying the understorey in each plot, measures of phylogenetic diversity and structure (Phylogenetic Diversity, PD; Net Relatedness Index, NRI; and Nearest Taxon Index, NTI) were calculated from a fully resolved seed-plant phylogeny obtained from a ITS-5.8S nrDNA dataset including original sequences from local plant material.ResultsThe resulting understorey phylogenetic tree allowed reliable estimation of PD, NRTI and NTI. Phylogenetic diversity was dependent on species richness and the strength of this relationship did not change along the gradient of overstorey tree species richness. Net Relatedness and Nearest Taxon indices were both mostly positive but non-significant, indicating random phylogenetic structure of the understorey. The presence of Quercus petraea, usually found in more favorable site conditions, was associated with a significant phylogenetic clustering at the species level.ConclusionsOverstorey species richness was not related to understorey phylogenetic structure, while species identity (Q. petraea) was associated with an increase in evolutionary relatedness in this forest layer, possibly due to competitive exclusion in favorable site conditions. Further studies should examine whether and how understory phylogenetic structure is affected by variables such as small scale variations in site conditions and presence of a shrub layer, both present but not accounted for in our model system. A better understanding of the role of these variables and their interaction may contribute to fill the current gap between the theoretical field of phylogenetic ecology and forest management.

Nitrogen (N) deposition, as one of the global change drivers, can alter terrestrial plant diversity and ecosystem function. However, the response of the plant diversity-ecosystem function relationship to N deposition remains unclear. On one hand, in the previous studies, taxonomic diversity (i.e., species richness, SR) was solely considered the common metric of plant diversity, compared to other diversity metrics such as phylogenetic and functional diversity. On the other hand, most previous studies simulating N deposition only included two levels of control versus N enrichment. How various N deposition rates affect multidimensional plant diversity-ecosystem function relationships is poorly understood. Here, a field manipulative experiment with a N addition gradient (0, 1, 2, 4, 8, 16, 32, and 64 g N m-2 yr-1) was carried out to examine the effects of N addition rates on the relationships between plant diversity metrics (taxonomic, phylogenetic, and functional diversity) and ecosystem production in a temperate steppe. Production initially increased and reached the maximum value at the N addition rate of 47 g m-2 yr-1, then decreased along the N-addition gradient in the steppe. SR, functional diversity calculated using plant height (FDis-Height) and leaf chlorophyll content (FDis-Chlorophyll), and phylogenetic diversity (net relatedness index, NRI) were reduced, whereas community-weighted means of plant height (CWMHeight) and leaf chlorophyll content (CWMChlorophyll) were enhanced by N addition. N addition did not affect the relationships of SR, NRI, and FDis-Height with production but significantly affected the strength of the correlation between FDis-Chlorophyll, CWMHeight, and CWMChlorophyll with biomass production across the eight levels of N addition. The findings indicate the robust relationships of taxonomic and phylogenetic diversity and production and the varying correlations between functional diversity and production under increased N deposition in the temperate steppe, highlighting the importance of a trait-based approach in studying the plant diversity-ecosystem function under global change scenarios.

Effect of cortical bone thickness and density on pullout strength of mini-implants: An experimental study

Cortical bone mapping improves finite element strain prediction accuracy at the proximal femur.

ABSTRACTObjectivesThe purpose of this observational clinical study was to evaluate the relationship between brain anatomical and volumetric changes in white matter, grey matter, and cerebral cortex thickness with the number of functional occlusal pairs present in the mouth.Material and MethodsThe number of functional occlusal pairs in 70 patients was counted and non-invasive brain analysis was performed using magnetic resonance imaging. The volume of grey matter, white matter, and thickness of the cortex in different areas of the brain were determined by SPM12 and CAT12 software. Multiple regression model corrected for multiple comparisons using FDR and Spearman correlation coefficient were calculated for statistical comparison.ResultsA total of 70 (39 male, 31 female) were analysed, with an average number of occlusal pairs of 10.21 (3.99). According to the Spearman correlation coefficient, a lower number of occlusal pairs was related to a reduction in white matter (right external capsule and posterior limb of the internal capsule), a reduction in grey matter (right temporal superior and medial gyrus and left cerebellum crus 1) and a reduction in thickness of the cerebral cortex (rostral anterior cingulated cortex of the right hemisphere and areas in the right and left hemisphere, especially in the frontal cortex). ConclusionsThe number of occlusal pairs is related to the volume of white matter, grey matter, and thickness of the cerebral cortex in areas of the brain that are directly involved in the onset and progression of Alzheimer’s disease and other dementias.