Corrigendum

Abstract

Plant Biotechnology JournalVolume 6, Issue 2 p. 210-211 Open Access Corrigendum First published: 03 January 2008 https://doi.org/10.1111/j.1467-7652.2007.00312.xAboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Dodo H. W., Konan K. N., Chen F. C., Egnin M. and Viquez O. M. (2007) Alleviating peanut allergy using genetic engineering: the silencing of the immunodominant allergen Ara h 2 leads to its significant reduction and a decrease in peanut allergenicity. The corresponding author's email should be hortense.dodo@aamu.edu. Figure 2 and its caption should be as follows: Figure 2Open in figure viewerPowerPoint Steps involved in the production of fertile transgenic peanut plants using pDK28, an Ara h 2 RNAi transformation vector. (a) Map of pDK28 binary plasmid mobilized into A. tumefaciens EHA105. (b) Germinated peanut embryos for preparation and co-culture of hypocotyl explants. (c) Selection of Kanamycin-resistant (Kr) shoots. (d) Rooting of regenerated Kr plants. (e) Transfer to soil and flowering. (f) Seeds from Kr peanut plants. In the section under ‘Regeneration of fertile transgenic peanut’, Figure 2 is cross-referenced incorrectly on page 137. The correct cross-reference should be ‘Kanamycin-sensitive plants turned white and died within 4 weeks, while kanamycin-resistant plants remained green and grew healthy (Figure 2c). A total of 59 kanamycin-resistant plants (Ara h 2-T0) were successfully rooted (Figure 2d) and transferred to pots in the Enconair growth chamber. Flowering occurred within 6 weeks following the transfer to the growth chamber (Figure 2e). Flowers were produced on both controls obtained by (i) direct peanut seed planting in soil (wild type) and (ii) tissue culture without Agrobacterium infection. The phenotype, plant growth and reproduction of the putative transgenic plants were indistinguishable from that of wild-type control. Peg elongated from the plants, and peanut pods were produced within 3–4 months (Figure 2f).’ In the section under ‘PCR and Southern hybridization confirmed the stable transformation of kanamycin-resistant peanut plants’, the second last sentence should read, ‘Analyses of the single digest (S) reveal that all the selected transgenic plants [plant #12 (19 pods), plant #23 (9 pods), plant #32 (4 pods), plant #39 (11 pods), plant #45 (7 pods), plant #55 (31 pods), plant #66 (3 pods) and plant #6 (25 pods)] carried each a single insert with a similar pattern of gene integration.’ Figure 5 should be labelled as follows: Figure 5Open in figure viewerPowerPoint Determination of Ara h 2 in peanut samples. (a) Sandwich ELISA shows the percentage of Ara h 2 in peanut total protein extracts. Numbers followed by the same letters are not statistically different at P < 0.05. (b) SDS-PAGE shows the protein profiles and (c) Western immunoblot analysis in crude peanut extracts to detect the presence of the Ara h 2 protein. Hybridization was performed using an Ara h 2 specific monoclonal antibody. About 0.5 µg purified Ara h 2 was loaded. WT, wild-type control sample; TCc, tissue culture non-transgenic control sample; (–), sample from a Southern negative plant; NT, not tested. Reference Dodo H. W., Konan K. N., Chen F. C., Egnin M. and Viquez O. M. (2007 ) Alleviating peanut allergy using genetic engineering: the silencing of the immunodominant allergen Ara h 2 leads to its significant reduction and a decrease in peanut allergenicity. Plant Biotechnol. J. 6, 135– 145. Google Scholar Volume6, Issue2February 2008Pages 210-211 FiguresReferencesRelatedInformation