Abstract
In this research, the subpopulations of human blood parameters including lymphocytes, monocytes, and granulocytes were determined byelectronic sizing in the Health Centre of UniversitiSains Malaysia. These parameters have beencorrelated with human blood characteristics such as age, gender, ethnicity, blood types, body mass index, medical history, number of chronic diseases, and type of chronic diseases; before and after irradiation with 15 W carbon dioxide laser (?=10,600 nm). The correlations were obtained byfinding patterns in changes of blood parameters using paired non-parametric tests, and an independent non-parametric test using the SPSS version 17. Irradiation of blood samples with carbon dioxide lasershowedsignificant changes in lymphocytes, monocytes, and granulocytes before and after irradiation.These analysis revealed that a significant increaseinlymphocyte before and after irradiation among different body mass index (p-value = 0.031).There is significant increase in monocyte before and after irradiation between medical history (p-value = 0.052), and number of chronic diseases (p-value = 0.022).And there is significant decrease in granulocyte before and after irradiation, among different body mass index (p-value = 0.021), and number of chronic diseases (p-value = 0.018).The correlation between changes in human blood parameters and a patient’s characteristics were very much correlated and can become a significant indicator for blood analyses.This study considered as a new finding for the increase inlymphocyte, monocyte, and the decrease in granulocyte by using low power Carbon dioxide laser radiation.
Highlights
The Carbon dioxide laser is widely used by surgeons and is the most commonly used medical lasers, and different from other medical lasers the action of this laser on tissues is clearly seen when it is used (Judy, 1995)
The analyses of white blood cell WBC parameter include: lymphocyte (LYM), monocyte (MID), and granulocyte (GRAN) parameters showed a significant increase in lymphocyte (p-value = 0.000) and in monocyte (p-value = 0.000) whereas a significant decrease in granulocyte (p-value = 0.000)
There is significant increase in mean lymphocyte before and after Carbon dioxide laser irradiation among different body mass index (p-value = 0.031), patients with underweight show the highest increase in mean lymphocyte (Table 2)
Summary
The Carbon dioxide laser is widely used by surgeons and is the most commonly used medical lasers, and different from other medical lasers the action of this laser on tissues is clearly seen when it is used (Judy, 1995). The Carbon dioxide laser offers a number of advantages for such applications. Postoperative pain usually is minimal to none www.ccsenet.org/mas (Pick & Colvard, 1993). This laser is absorbed strongly by water that formed > 80% of soft tissues (Oswal, et al, 1988; Apfelberg, et al, 1981a, 1984b; Kaplan & Giler, 1984; LoCicero, et al, 1985). Most of the irradiation energy of a Carbon dioxide laser is absorbed by the tissue surface, and little penetrates to cause damage in deeper tissues (Kumazawa, et al, 1992)
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