Abstract
In this study we combined μXRF and LA-ICP-MS on the very same human femoral head sample. Obtained spatial elemental distributions within bone and cartilage tissues are accompanied by absolute quantitative data.
Highlights
Investigation of biological tissues o en requires obtaining comprehensive chemical elemental information
Local elemental distributions were obtained from the measurement areas, which were chosen such as to include bone, articular cartilage tissue and the metabolically active area of tidemark – the interface between calci ed and non-calci ed cartilage; the elemental maps of 15 mm and 41 mm cuts are shown on Fig. 2 and 3, respectively
These gures show the very same area within each slice imaged by microbeam X-ray fluorescence spectrometry (mXRF) and LA-ICP-MS
Summary
Investigation of biological tissues o en requires obtaining comprehensive chemical elemental information. Due to its inorganic components – it contains a mineral phase (hydroxyapatite), is a tissue especially suitable for elemental analysis. The interface between mineralized and hyaline cartilage is called tidemark. It is known that the tidemark is the site of metal accumulation, e.g. zinc and lead, and several elemental imaging experiments have been devoted to this topic on animal or human cartilage samples.[7,8,9,10] Compared to bone, hyaline cartilage is substantially less investigated in terms of elemental composition. There are some imaging approaches to visualize hyaline cartilage, such as uorescent microscopy, contrastenhanced scanning electron microscopy, computed tomography and magnetic resonance imaging,[11,12] the imaging experiments involving the elemental distribution are scarce
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