Abstract
2012. Photothermal confocal spectromicroscopy of multiple cellular chromophores and fluorophores. Biophys. J. 102:672–681. Authors update names to: Dmitry A. Nedosekin, Ekaterina I. Galanzha, Srinivas Ayyadevara, Robert J. Shmookler Reis, and Vladimir P. Zharov. Authors also acknowledge the help of Dr. Alexandru S. Biris (University of Arkansas for Medical Sciences) in preparation of gold film samples. Photothermal Confocal Spectromicroscopy of Multiple Cellular Chromophores and FluorophoresNedosekin et al.Biophysical JournalFebruary 08, 2012In BriefConfocal fluorescence microscopy is a powerful biological tool providing high-resolution, three-dimensional (3D) imaging of fluorescent molecules. Many cellular components are weakly fluorescent, however, and thus their imaging requires additional labeling. As an alternative, label-free imaging can be performed by photothermal (PT) microscopy (PTM), based on nonradiative relaxation of absorbed energy into heat. Previously, little progress has been made in PT spectral identification of cellular chromophores at the 3D microscopic scale. Full-Text PDF Open Archive
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