Correction of glycemic and oxidative stress sensors in streptozotocin induced diabetic rat model: Impact of dose dependent study of n-butanol solvent fraction of hydro-methanol extract of banana flower (Musa balbisiana).

Abstract

The experiment focused the most effective dose out of 2.5, 5, and 10mg/100g body weight/day for 4weeks of n-butanol fraction from the pretested promising hydro-methanol extract of Musa balbisiana flower to manage the streptozotocin-induced diabetes in rat and to minimize the bioburden of the body by excluding the inactive components from the said extract. Fraction treatment exhibited an outstanding recovery in fasting blood glucose, glycated hemoglobin, serum insulin levels, carbohydrate metabolic, antioxidant enzymes activities, expression of their marker genes, and apoptotic gene in diabetic rat. Flow-cytometric study of pancreatic β-cell population was significantly recovered in fraction-treated groups which was also supported by histological study. The moderate dose, that is, 5mg dose showed maximum efficacy in respect to other doses. The high dose, that is, 10mg dose is not significantly effective than 5mg dose may be due to the desensitization of the target organ's orphan receptor(s). PRACTICAL APPLICATIONS: The work includes the role of M. balbisiana on diabetes management. Result focused that M. balbisiana flower can able to rectify the diabetic condition and recovered the β cell population. M. balbisiana flower is a very common vegetable to people, use mostly in their daily meal. The experiment showed positive results to cure diabetes in the long-term. Moreover, this study is potential for drug development using nutraceutical(s) isolation. The pharmaceutical companies may develop an antidiabetic drug from the concerned plant part after successful clinical trial.