Correction: Changes in histone acetylation and methylation that are important for persistent but not transient expression of CCR4 in human CD4+ T cells

Abstract

An error was introduced in Figure 1 in this article. The correct figure is shown below. Patterns of receptor expression and cytokine production in cells activated in vitro. Naïve (CD45RA+CD62L+CXCR3− CCR4−) CD4+ T cells from cord blood (Supporting Information Fig. 1) were stimulated with plate-bound anti-CD3 and soluble anti-CD28 in non-polarizing, Th1, or Th2 conditions. (A) On day 12, cells were stained with FITC-anti-CD4 and PE-anti-CXCR3 or FITC-anti-CD4 and PE-anti-CCR4 antibodies or stimulated with the leukocyte activation cocktail for 6 h, fixed, permeabilized and stained with FITC-anti-CD4 and APC-anti-IFN-γ or FITC-anti-CD4 and APC-anti-IL-4 antibodies before analysis. Quadrants were drawn based on the staining with isotype controls (Supporting Information Fig. 2) and the percentages of cells staining for chemokine receptor (left panel) or cytokine (right panel) are displayed. (B) Polarized cells were stimulated with PMA and ionomycin and the mRNA levels for chemokine receptor and cytokine genes were quantified using real-time RT-PCR. Values were normalized to GAPDH and then to the results for naïve cells. Data in (A) are from one donor, representative of five donors/experiments. Data in (B) are means+SEM of pooled results from three donors/experiments where RNA analysis was done together with receptor and cytokine staining, including the donor displayed in (A). **p<0.01 versus all other samples using Student's t-test.