Abstract
CuxO-NiO nanocomposite film for the non-enzymatic determination of glucose was prepared by the novel modifying method. At first, anodized Cu electrode was kept in a mixture solution of CuSO4, NiSO4 and H2SO4 for 15 minutes. Then, a cathodization process with a step potential of -6 V in a mixture solution of CuSO4 and NiSO4 was initiated, generating formation of porous Cu-Ni film on the bare Cu electrode by electrodeposition assisted by the release of hydrogen bubbles acting as soft templates. Optimized conditions were determined by the experimental design software for electrodeposition process. Afterward, Cu-Ni modified electrode was scanned by cyclic voltammetry (CV) method in NaOH solution to convert Cu and Ni nanoparticles to the nano-scaled CuxO-NiO film. The electrocatalytic behavior of the novel CuxO-NiO film toward glucose oxidation was studied by CV and chronoamperometry (CHA) techniques. The calibration curve of glucose was found linear in a wide range of 0.04–5.76 mM, with a low limit of detection (LOD) of 7.3 µM (S/N = 3) and high sensitivity (1.38 mA mM-1 cm-2). The sensor showed high selectivity against some usual interfering species and high stability (loss of only 6.3 % of its performance over one month). The prepared CuxO-NiO nanofilm based sensor was successfully applied for monitoring glucose in human blood serum and urine samples.
Highlights
glucose oxidase (GOD) is quite stable compared to other enzymes, glucose sensors based on GOD are always exposed to a possibility of thermal and chemical deformation during fabrication, storage or use
Various nanostructured materials have been proposed as new opportunities to develop novel non-enzymatic glucose sensors [6] such as carbon nanotubes [11], metals [12,13], metal oxides such as NiO [14,15,16,17] or Cu2O [18], metal composites such as Cu-Ni [19], etc
Cu@Ni/MWCNT nanocomposite was prepared for simultaneous electrochemical determination of guanine and adenine [22], Cu@Ni core–shell nanoparticles/reduced graphene oxide nanocomposite for non-enzymatic glucose sensor [23], and non-enzymatic multispecies sensor based on Cu-Ni nanoparticle dispersion on doped graphene [19]
Summary
GOD is quite stable compared to other enzymes, glucose sensors based on GOD are always exposed to a possibility of thermal and chemical deformation during fabrication, storage or use. Glucose dehydrogenase enzyme showed the problem of effectiveness from interfering species like AA, DA, etc. Non-enzymatic glucose sensors have received significant interest due to their advantage of thermal and chemical stability [7]. Various nanostructured materials have been proposed as new opportunities to develop novel non-enzymatic glucose sensors [6] such as carbon nanotubes [11], metals [12,13], metal oxides such as NiO [14,15,16,17] or Cu2O [18], metal composites such as Cu-Ni [19], etc. Cu@Ni/MWCNT nanocomposite was prepared for simultaneous electrochemical determination of guanine and adenine [22], Cu@Ni core–shell nanoparticles/reduced graphene oxide nanocomposite for non-enzymatic glucose sensor [23], and non-enzymatic multispecies sensor based on Cu-Ni nanoparticle dispersion on doped graphene [19]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Electrochemical Science and Engineering
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
