Abstract
The mortality of adults, eggs, larvae and pupae of the cowpea bruchid, Callosobruchus maculatus (F.) and the bean bruchid, Acanthoscelides obtectus (Say) in 100% nitrogen (N 2) atmosphere at 25 and 32°C, respectively, and at 70±5% relative humidity, was investigated. Irrespective of temperature, all adults of both bruchids were killed within 1 day of exposure to pure N 2 atmosphere. All eggs were killed in 5 days at 25°C and in 3 days at 32°C. Complete mortality of larvae and pupae was observed in 9 days at 25°C and in 5 days at 32°C, except with young larvae of A. obtectus, which were all killed in 5 days at 25°C and in 3 days at 32°C. Exposure to an atmosphere of 100% N 2 could therefore provide a rapid means of disinfestation of stored grain legumes from C. maculatus and A. obtectus.
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