Abstract
1. The incorporation of [ 14C]phenylalanyl-tRNA into peptide was found to be the same in ribosomes from kidney of control and nephrotic rats that were incubated with excess poly(U) and a control liver transferase preparation. 2. The pH 5 supernatant fraction was prepared from kidney of control and nephrotic rats and incubated with control liver ribosomes. The incorporation of [ 14C]phenylalanyl-tRNA into peptide was two times higher with the nephrotic rat kidney preparation. 3. The increased incorporation could not be attributed to pH 7.8 alkaline ribonuclease activity nor its inhibitor but the increase was associated with aminoacyl-tRNA binding factor (transferase I) activity. 4. Kidney aminoacyl-tRNA binding factor (transferase I) was separated from peptidyl-tRNA translocation factor (transferase II) by Sephadex G-200. The incorporation of [ 14C]phenylalanyl-tRNA into peptide was measured using fractions containing transferase I obtained from control and nephrotic kidney and supplemented with control liver transferase II factor. Similarly fractions containing transferase II obtained from control and nephrotic kidney were supplemented with control liver transferase I factor. A significant increase was found in the specific activity of fractions containing transferase I from nephrotic kidney.
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