Abstract
Reservoir souring is the increased sulfide concentration in oil production fluid, and it's the major problem faced during the secondary oil recovery process. Recently, nitrate injection emerged as a souring control strategy by inhibiting sulfate-reducing bacteria that cause souring. However, this strategy showed limited success due to the complete reduction of nitrate into nitrogen. The present study revealed that souring could be controlled by incomplete reduction of nitrate to nitrite at high temperatures (>55 °C) in Indian oil fields. Present study showed that, at 55 °C incomplete nitrate reduction led to the accumulation of nitrite and control of souring. This incomplete nitrate reduction was due to under-expression of the nitrite reductase gene at 55 °C. At 37 °C, nitrate and nitrite reductase genes were expressed consecutively, but at 55 °C, nitrite reductase gene expression decreased significantly and led to nitrite accumulation.
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