Contribution of non-ADH pathways to ethanol oxidation in hepatocytes from fed and hyperthyroid rats: Effect of fructose and xylitol

Abstract

The metabolism of (1R)[1- 3H]ethanol, [2- 3H]lactate or [2- 3H]xylitol was studied in hepatocytes from fed or T 3-treated rats in the presence or absence of fructose or xylitol. The yields of tritium in ethanol. lactate, water, glycerol and glucose were determined. A simple model, describing the metabolic fate of tritium from these substrates is presented. The model allows estimation of the ethanol oxidation rate by the non-alcohol dehydrogenase pathways from the relative yield of tritium in water and glucose. The calculations are based on a comparison of the fate of the 1- proR-hydrogen of ethanol and the hydrogen bound to carbon 2 of lactate (or xylitol) under identical condition. In our calculations we have taken into account that the reactions catalyzed by lactate dehydrogenase and alcohol dehydrogenase are reversible and that lactate or ethanol labelled during the metabolism of the other tritiated substrates will contribute to the tritium found in water. The contribution of non-ADH pathways to ethanol oxidation varied from 10 to 50% and was correlated to changes in the lactate/pyruvate ratio from 80 to 500. In T 3- treated rats the activity of non-ADH pathways were greater than in fed rats for the same lactate/pyruvate ratio.