Continuous release of O2−/ONOO− in plasma‐exposed HEPES‐buffered saline promotes TRP channel‐mediated uptake of a large cation

Abstract

AbstractAlthough the externally controllable extracellular supply of the short‐lived reactive oxygen and nitrogen species, such as O2•−, •NO, and ONOO−, could potentially manipulate cellular functions, their simple administration to cells is likely to be ineffective due to their rapid deactivation. In this study, we found a method of a continuous supply of O2•−/ONOO− over a few minutes, which is triggered by irradiation of a nonequilibrium atmospheric pressure plasma to commonly used organic buffers (e.g., 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid, HEPES). In addition, a continuous low‐dose O2•−/ONOO− supply was shown to induce a physiologically relevant Ca2+ response and subsequently the uptake of a large cation mediated by transient receptor potential channel family member(s). Our results provide a novel approach to the continuous O2•−/ONOO− supply, requiring controllable and mass‐volume treatments.