Abstract
Arylsulfatases are determined, evenin weakly acidic solution, by a direct and continuous kinetic method using new coumarin-derived sulfates as substrates. After enzymatic hydrolysis, the substrate dissociates to form intensely colored and strongly fluorescent phenolates, with absorption maxima ranging from 383 to 497 nm, and fluorescence emission maxima between 470 and 577 nm. Rates of enzymatic hydrolysis, optimum pH values and detection limits of arylsulfatase from Aerobacter aerogenes and Patella vulgata are determined.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
