Construction of ratiometric fluorescence sensor and test strip with smartphone based on dual-emission carbon dots for the specific detection of chlortetracycline.

Abstract

Concerns about environmental and food contamination caused by chlortetracycline (CTC) residues have prompted people to explore efficient and convenient CTC monitoring platforms. However, the reported fluorescent probes generally fail to selectively detect CTC due to the structural similarity of tetracycline antibiotics. Herein, an intrinsic dual-emission carbon dots (D-CDs) ratiometric fluorescence sensor was prepared for highly sensitive and selective determination of CTC over other tetracyclines by one-step synthesis. The sensor exhibited a significant fluorescence enhancement at 425 nm after introducing CTC. The fluorescence "turn on" of the sensing system is due to aggregation-induced emission (AIE) phenomenon formed by hydrogen bonds and π conjugation promoting the specific recognition of CTC by D-CDs. The linear detection varied from 0.98 to 143.67 ng mL-1 with a low limit of detection (LOD) of 1.29 ng mL-1 (R2 = 0.998), which was lower than most reported in the literature. The D-CDs sensor was applied to detect CTC in spiked milk, blocked normal human serum, and fish samples with recoveries of 95.5-104.2% and relative standard deviations (RSDs) of 2.6%. Particularly, D-CDs based test papers with a smartphone were prepared for portable and visual detection of CTC by analyzing the various color changes of RGB of fluorescence color, with an LOD of 7.18 ng mL-1 (R2 = 0.9909). The fluorescence sensor designed in this work could be used as a rapid tool with high performance and selectivity for monitoring control in foods.