Abstract
SUMMARY: The following requirements should be met by polyploid yeast sets optimally suited for gene dosage studies: isogenicity; homozygous wild-type genetic background; amenability to genetic analysis and preferably normal segregation of the mating type alleles; ploidy number as high as possible, but compatible with the above requirements. The present study shows that tetraploid yeast sets which meet all these requirements may be developed with relative ease by the controlled cross procedure. The aa and αα diploids which constitute the parents of the crosses are obtained either by the selection of cells appearing spontaneously in haploid cultures upon prolonged cultivation (14 clones have thus been obtained), or by the choice of appropriate genotypes among the progeny of tetraploid clones. This paper reports the segregation data of a typical complete genetic analysis in which tetraploid clones are reduced by two successive meioses to the haploid state; together with first meiosis segregation data from about seventy tetraploid clones.
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