Abstract
To understand the mechanism of benzothiadiazole (BTH)-induced susceptible rubber clone resistance to powdery mildew on gene level, a differentially expressed cDNA library was constructed by suppression subtractive hybridization (SSH) with rubber Reyan 7-33-97 clone. The constructed cDNA library was high integrity through detection of the critical processes of SSH, such as efficiency of adaptor connection, subtraction and conversion, as well as the type of recombinant genes. The positive rate was 99% after identification with random 400 white spots. The size of the cDNA clone inserted fragments was various but most in 400 bp - 1000 bp. There were 23 cDNA sequences matching the function of energy and basic metabolism, signal transduction, membrane and transport, secondary metabolism and so on after detection of the 42 positive clone sequences selected randomly from the cDNA library and comparison on nucleic acid sequences in Genbank. 7 ESTs were logged in Genbank and accession numbers were GW873071 and GW874604- GW874610. The results implicated that BTH could effectively induced rubber tree resistance to powdery mildew through increasing expresses of defense-related genes in leaves of rubber tree susceptible clone. It should provide a new approach for rubber disease management.
Highlights
Rubber tree (Hevea brasiliensis) is a very important tropical industrial crop
To understand the mechanism of benzothiadiazole (BTH)-induced susceptible rubber clone resistance to powdery mildew on gene level, a differentially expressed cDNA library was constructed by suppression subtractive hybridization (SSH) with rubber Reyan 7-33-97 clone
To understand the mechanism of BTH induced rubber tree resistance to powdery mildew in molecular knowledge, a cDNA library of BTH-induced resistance to the disease of rubber tree was constructed through SSH
Summary
Powdery mildew disease caused by Oidium heveae is one of the most important leaf diseases and impacts severely to the growth and latex production of rubber trees [1]. To control this disease, rubber resistance clone breeding and chemical control were employed generally [2]. The mechanism of BTH induced resistance is activation of the plant defense genes and expression resistance-related proteins or enzymes [10]. No report has been found so far on BTH induced defense genes in rubber tree susceptible clones against the disease. To understand the mechanism of BTH induced rubber tree resistance to powdery mildew in molecular knowledge, a cDNA library of BTH-induced resistance to the disease of rubber tree was constructed through SSH
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
