Conservation implications of the genetic and ecological distinction of Tursiops truncatus ecotypes in the Gulf of California

Abstract

The demand for live bottlenose dolphins for commercial use is growing in Mexico, making the need for stock assessment and management ever more essential given their protected status. Tursiops truncatus is known to exhibit high levels of phenotypic polymorphisms. In the Gulf of California (GC), coastal and offshore ecotypes have been identified based on morphological, behavioral and ecological evidence, including different prey and habitat preferences. However, the extent to which this ecological and phenotypic variation is genetically correlated is unknown. Here we assess this correlation in GC bottlenose dolphins classified as coastal or offshore based on habitat, morphological and trophic evidence. Significant ( p < 0.0001) haplotype heterogeneity (exact test) and genetic differentiation ( F ST = 0.069) were found in the mitochondrial control region, indicating some reproductive isolation between ecotypes. As elsewhere, coastal dolphins were less diverse than offshore. Phylogenetic analyses revealed paraphyletic coastal and offshore lineages and no evidence of lineage sorting, possibly due to recent isolation or gene flow. This is the first time that genetic, morphological and stable isotope evidence has been used to recognize ecotypes as different stocks for management purposes in bottlenose dolphins. Our results indicate that diversifying forces are shaping their phenotypic and genetic variation in the GC. Management and conservation efforts in this strategic region should aim to preserve these forces.