Abstract
The performances of porous silicon optical biosensors depend strongly on the arrangementof the biological probes into their sponge-like structures: it is well known that in this casethe sensing species do not fill the pores but instead cover their internal surface. In thispaper, the direct imaging of labelled proteins into different porous silicon structures byusing a confocal laser microscope is reported. The distribution of the biological matter inthe nanostructured material follows a Gaussian behaviour which is typical of the diffusionprocess in the porous media but with substantial differences between a porous siliconmonolayer and a multilayer such as a Bragg mirror. Even if semi-quantitative, the resultscan be very useful in the design of the porous silicon based biosensing devices.
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