Abstract
This study describes a novel approach to generate conditionally immortalized preadipocyte cell lines from white adipose tissue (IMWAT) that can be induced to differentiate into white adipocytes even after expansion in culture. Such adipocytes express markers of white fat such as peroxisome proliferator-activated receptor gamma and aP2 but not brown fat markers, have an intact insulin signaling pathway, and express proinflammatory cytokines. They can be readily transduced with adenoviral vectors, allowing them to be used to investigate the consequences of the depletion of specific adipocyte factors using short hairpin RNA. This approach has been used to study the effect of reduced expression of the nuclear receptor corepressor receptor interacting protein 140 (RIP140), a regulator of adipocyte function. The depletion of RIP140 results in changes in metabolic gene expression that resemble those in adipose tissue of the RIP140 null mouse. Thus, IMWAT cells provide a novel model for adipocytes that are derived from preadipocytes rather than fibroblasts and provide an alternative system to primary preadipocytes for the investigation of adipocyte function.
Highlights
This study describes a novel approach to generate conditionally immortalized preadipocyte cell lines from white adipose tissue (IMWAT) that can be induced to differentiate into white adipocytes even after expansion in culture
A number of cell types, including myoblasts, have been derived from this mouse model [13,14,15,16]; these proliferate at permissive temperature (33jC) in the presence of interferon g but can Abbreviations: GFP, Green Fluorescent Protein; Interleukin 6 (IL-6), interleukin 6; IMBAT, immortalized brown adipocyte; IMWAT, immortalized white adipocyte; LPS, lipopolysaccharide; PRDM, PR domain containing 16; shRNA, short hairpin RNA; Rb, Retinoblastoma; Tumor necrosis factor-a (TNF-a), tumor necrosis factor-a; Ucp1, Uncoupling Protein 1
We have shown that conditionally immortalized white preadipocytes derived from the H-2kb-tsA58 transgenic mouse retain the ability to differentiate into white adipocytes
Summary
This study describes a novel approach to generate conditionally immortalized preadipocyte cell lines from white adipose tissue (IMWAT) that can be induced to differentiate into white adipocytes even after expansion in culture. Such adipocytes express markers of white fat such as peroxisome proliferator-activated receptor g and aP2 but not brown fat markers, have an intact insulin signaling pathway, and express proinflammatory cytokines. SV40 T-antigen functions by deactivating proteins, including Retinoblastoma (Rb), and recent studies have shown that Rb null preadipocytes differentiate preferentially into brown adipocytes [9] It appears that members of the E2F family, themselves regulated by Rb, have roles in adipogenesis [10]. This article is available online at http://www.jlr.org
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
