Abstract
Abstract The use of cross-flow filtration (CFF) to concentrate hydrophobic material in marine colloids was investigated. The efficiencies of CFF to fractionate and concentrate colloids from water samples were evaluated by determining blanks and mass balances of hydrocarbons, acyl lipids, ketones and alcohols in marine colloids from algal cultures and seawater. It was possible to minimize artifact formation in the CFF system through the use of Teflon components, by not recycling retentate fractions, and by optimizing cleaning procedures and filtration conditions. The hydrocarbon and total lipid blanks in the CFF system were 0.5 μg and 7 μg respectively. For the major lipid classes in both algal culture and seawater samples, the mass balances on the 0.45 μm filter and the 10 K Dalton cut-off filter were 80–120%. This indicates that contamination from the CFF system and adsorption onto the components have been minimized.
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