Computer-assisted quantitative framework for whole slide cervical image grading driven by time series features

IntroductionA liquid-based cytology test was introduced for cervical cancer screening in the 2000s worldwide. However, the concordance of diagnostic findings between the liquid-based cytology test and cervical biopsy has not been fully investigated, especially the overall failure rate on the diagnosis of cervical cancer and high-grade squamous intraepithelial lesion (HSIL) by cytology testing. The aim of this retrospective study was to investigate the concordance between ThinPrep cytology and histology test in the diagnosis of cervical cancer and HSIL in HPV-positive women.MethodsThinPrep cytology test was performed in 2,472 HPV-positive women. Out of 2,472 HPV-positive women, the cervical biopsy was concurrently performed in 1,533 women. Data on the HPV type and the diagnostic findings of the ThinPrep cytology test and cervical biopsy were collected from our hospital electronic database. The concordance of diagnostic findings between cytology and histology was compared.ResultsThe rate of agreement in the diagnosis of the low-grade squamous intraepithelial lesion (LSIL) or HSIL between cervical biopsy and ThinPrep cytology test was 58 or 49%. The overall false negative rate in the diagnosis of cervical cancer and HSIL by ThinPrep cytology test was 6%. However, when considering the total number of HPV-positive women diagnosed with cervical cancer (n = 36) and HSIL (n = 117) by cervical biopsy, we found that a significant number of HPV-positive women with cervical cancer (n = 12, 33%), or women with HSIL (n = 77, 66%) were failed to be diagnosed by the ThinPrep cytology test. These HPV-positive women were either diagnosed with cervical infection or ASCUS, or LSIL.DiscussionOur data demonstrated that in order to ensure an accurate diagnosis, an immediate cervical biopsy in women with cervical infection or ASCUS or LSIL should be strongly recommended in clinical practice.

Objectives:To determine the application value of thinprep cytologic test (TCT) combined with serum carbohydrate antigen 153 (CA153) and carbohydrate antigen 50 (CA50) detection in the early diagnosis and screening of cervical cancer and precancerous lesions.Methods:A total of 187 females with cervical lesions admitted to Shanghai 7th People’s Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from January 2017 to December 2018 were selected and divided into two groups: the cervical cancer group and the cervical precancerous lesion group, with 16 cases in the cervical cancer group and 171 cases in the cervical precancerous lesion group (cervical precancerous lesions were divided into 63 cases of the CNI group, 59 cases of the CNII group and 49 cases of the CNIII group). During the same period, 106 healthy females were selected as the healthy group. The serum tumor markers CA153 and CA50 of all subjects were detected by chemiluminescence method; The diagnostic value of TCT combined with serum CA153 and CA50 in cervical cancer and precancerous lesions was analyzed with colposcopy pathological diagnosis results as gold standard; ROC curve was drawn to evaluate the diagnostic value of serum TCT, CA153 and CA50 in cervical cancer and precancerous lesions.Results:The levels of serum CA153 and CA50 in the cervical cancer group were significantly higher than those in the cervical precancerous lesion group and the healthy group (p< 0.05), and the levels of serum CA153 and CA50 in the cervical precancerous lesion group were significantly higher than those in the healthy group (p< 0.05). The sensitivity of TCT, serum CA153 and serum CA50 in the single detection of cervical cancer and precancerous lesions was 95.93%, 97.54% and 96.00%, the specificity was 59.41%, 60.23%, 60.12%, the accuracy was 74.74%, 75.77%, 75.43%, the positive predictive value was 62.03%, 63.64%, 63.10%, and the negative predictive value was 96.22%, 97.17% and 95.28%, respectively. The sensitivity, specificity, accuracy, positive predictive value and negative predictive value of TCT combined with serum CA153 and CA50 were 96.77%, 73.19%, 85.67%, 80.21% and 95.28%, respectively. ROC curve showed that the area under the curve (AUC) of TCT and serum CA153 and CA50 in the single detection of cervical cancer and precancerous lesions was 0.791, 0.864 and 0.787, respectively, the AUC of combined detection of TCT and serum CA153 and CA50 in patients with cervical cancer and precancerous lesions was 0.877, which was significantly higher than that of single detection (p< 0.05).Conclusions:TCT combined with serum CA153 and CA50 has been reported as a treatment regimen with high accuracy, which has a high diagnostic efficiency for early diagnosis of cervical cancer and precancerous lesions, and can significantly improve the sensitivity.

Objective To explore the value of cervical lesions screened with white acetate solution. Methods Four hundred and eighty- five patients who were suspected with cervical lesion during Janurary to March 2016 were enrolled in this study. The patients were asked about the medical history, filled the registration form, and performed routine gynecological examinations with white acetate solution, thinprep cytology test (TCT) and vaginal endoscopic biopsy. The value of white acetate solution in clinic was assessed. Results In 485 patients, 450 patients (92.78%) were pathologically diagnosed of inflammation (92.78%), 24 patients (4.95%) were diagnosed of low-grade squamous intraepithelial lesion(LSIL), 10 patients (2.06%)were diagnosed of high- grade squamous intraepithelial lesion (HSIL), and one patient (0.21%) was diagnosed of cervical cancer. The detection rate of white acetate solution and TCT were gradually increased with the increase of the degree of cervical lesions. The detection rate of white acetate solution in the inflammation was 1.33%, in LSIL was 91.67%, in HSIL was 9/10, and in cervical cancer was 1/1. The detection rate of TCT in the inflammation was 8.44%, in LSIL was 66.67%, in HSIL was 6/10, and in cervical cancer was 1/1. The sensitivity, specificity and coincidence rate of the diagnostic results with white acetate solution were 84.21%, 99.33%, 98.14%, and with TCT were 62.30%, 93.63%, 89.69%. There were significant differences (P <0.05). Conclusions Cervical lesions screened with white acetate solution is good in sensitivity and specificity, which is an effective way for screening cervical cancer. Key words: Uterine cervical diseases; Diagnostic self evaluation; Pathology, clinical; White acetate solution; Thinprep cytology test

BackgroundThis study sought to systematically analyze the clinical diagnostic value of tumor markers combined with ThinPrep cytologic test (TCT) and human papillomavirus (HPV) deoxyribonucleic acid (DNA) detection for cervical cancer and pre-cancerous lesions. However, there is a lot of controversy in the field of TCT + HPV-DNA. Many people have mixed opinions on the accuracy of TCT + HPV-DNA, and there is no unified opinion. Therefore, it is necessary to further confirm the significance of this combined detection method in the early diagnosis of cervical cancer by applying meta method.MethodsThe Cochrane Library, PubMed, Web of Science, Embase, Chinese Biomedical Literature Database (CBM) databases were searched to retrieve studies. To assess the methodological quality of each study and potential risk of bias, QUADAS-2 Guidelines were used to evaluate the quality of all articles that met the inclusion criteria and data extraction of the included articles were performed, and a meta-analysis was performed of the included studies using Review Manager 5.2 software.ResultsA total of 5 studies were included in the study, and a total of 2,778 patients were included in the study, and there was no significant publication bias observed. The meta-analysis showed that there was a statistical difference in terms of the accuracy of the tumor markers combined with TCT in the detection of cervical cancer. The results were as follows: the pooled sensitivity (SEN) was 0.86 (95% CI: 0.75–0.93); the combined specificity (SPE) was 0.79 (95% CI: 0.57–0.92); the diagnostic performance of combined with thin-layer liquid-based cytology and HPV DNA detection in the diagnosis accuracy of cervical cancer by summary receiver operating characteristic (SROC) curve analysis, result showed excellent diagnostic accuracy, with a combined area under the curve (AUC) of 0.90 (95% CI: 0.87–0.92).DiscussionTumor markers are important for the early diagnosis of cervical cancer. Combining the tumor markers with TCT and HPV DNA detection effectively improved the detection rate.

Objective To investigate the screening value of the thinprep cytological test(TCT) detection combined with human papilloma virus(HPV) hybrid capture Ⅱ(HC-Ⅱ) for cervical carcinoma. Methods From January 2003 to January 2005, the thinprep cytological test and hybrid capture Ⅱ were applied to screen cervical carcinoma in 435 cases in the Department of Obstetrics and Gynecology in Hexian Memorial Hospital. The thinprep cytological test was applied to detect cervical carcinoma cytology, and hybrid capture Ⅱ was applied to detect human papilloma virus. According to the detection results, all cases were divided into 4 groups, group of HPV(-), TCT(-), group of HPV(+ ), TCT(-), group of HPV(-), TCT(+ ), group of HPV(+ ), TCT(+ ). A follow-up of 3 years had been carried out, and different groups were checked by the thinprep cytological test and human papilloma virus hybrid capture Ⅱ at different interval times, and colpomicroscope, biopsy and endocervical curettage (ECC) would also be used if necessary. Determinant elements of cytology occurrence were evaluated by the Logistic regression analysis. Results The sensitivity, specificity, efficiency, positive predictive value (PPV), negative predictive value(NPV), Kappa values of the thinprep cytological test and human papilloma virus hybrid capture Ⅱwere 85.9%, 96.2%, 94.7%, 79.7%, 97.5%, 0.7959, and 92.2%, 80.1%, 81.8%, 44.4%, 98.3%, 0.4996. The group of HPV(+ ), TCT(-), and the group of HPV(-), TCT(+ ) had higher risk of pathological changes of cervical carcinoma (RR=45.033, 95%CI 17.140~118.313; RR=235.886, 95% CI 70.694~487.088) compared with the group of HPV(-), TCT(-), and the group of HPV(+ ), TCT(+ ) had distinct risks (RR=464.400, 95% CI 105.162~950.806). Conclusion The detection of sensitivity and specificity by the combination of the thinprep cytological test with human papilloma virus hybrid capture Ⅱ can be enhanced, which could meet the requirement of screening the early stage of cervical carcinomas and precancerous changes. Key words: thinprep cytologic test; hybrid capture Ⅱ; cervical carcinoma; screening

Cervical cancer is one of the most prevalent female cancer types in developing countries. ThinPrep cytological test (TCT) and human papillomavirus (HPV) detection are canonical screening methods for cervical cancer currently. However, there are limitations to these techniques. The aim of the present study was to identify efficient and practical methods for the screening of cervical intraepithelial neoplasia (CIN) and carcinoma. Residual PreservCyt specimens were obtained from 1,000 women who were admitted between August 2013 and December 2015. TCT, human telomerase RNA component (h-TERC) fluorescent in situ hybridization (FISH), MYC-specific FISH and surface plasmon resonance (SPR)-HPV genotyping were performed, followed by histopathology for those patients with positive results in any of the four tests. As a result, 106, 64, 56 and 112 patients were positive in the TCT, h-TERC, c-MYC and SPR-HPV tests, respectively, resulting in 213 being scheduled for histopathology; inflammation was identified in 159 patients, CIN I in 31, CIN II in 14, CIN III in seven and invasive cervical cancer in two patients. Using histopathology as the gold standard, TCT exhibited the highest sensitivity (87.04%), while h-TERC analysis had the highest specificity (81.76%). Parallel tests demonstrated that the Youden's index of TCT + h-TERC was the highest (0.49), while the serial analysis reported that TCT + HPV had the highest Youden's index (0.53) compared with any of the biomarkers alone (TCT, 0.50; HPV, 0.29; h-TERC, 0.47). In conclusion, dual positive TCT and HPV may be an efficient approach for basic screening of cervical lesions. h-TERC amplification may serve as an auxiliary test to improve the specificity.

Objective This study aimed to explore the clinical value of human papilloma virus (HPV) E6/E7 mRNA tests in identifying precancerous lesions of the uterine cervix- cervical intraepithelial neoplasia 2 or more CIN2+ (CINⅡand CINⅢ). Methods This study is a cross-sectional survey design, between December 2011 to December 2013.The specimens were collected from the First People's Hospital of Huizhou and the Third People's Hospital of Huizhou in Department of Obstetrics and Gynecology outpatient and inpatient of cervical disease suspected patients, with thin-prep cytologic test (TCT) and histopathological results as reference, detected 345 patients of exfoliated cervical epithelial cells by using the branched DNA (b-DNA) technology to evaluate the application value of high risk HPV E6 / E7mRNA in the clinical diagnosis of CIN.Using spss 19.0 software for data analysis. Results (1)Compared with TCT, the positive rate of E6/E7 mRNA in 325 samples were grading by cytology as follows: no intraepithelial lesion cells (NILM) 21.1% (40/190), atypical squamous cells (ASC) 38.5% (15/39 ), low-grade squamous intraepithelial lesions (LSIL) 76.9% (30/39), atypical squamous cells can not exclude high-grade intraepithelial lesions (ASC-H) (8/10), high-grade squamous intraepithelial lesions (HSIL) 72.3% (34/47), TCT grades and HPV E6/E7 mRNA positive rate showed linear association (χ2=67.654, P<0.01; r=0.497, P<0.01); and with HPV E6/E7 mRNA copy number was also relevant (r=0.511, P<0.01). (2) Compared with pathological results, the positive rate of E6/E7 mRNA in 164 women samples were grading by pathology as follows: with NILM was 27.8%(10/36), with CIN Ⅰ was 65.9%(29/44), with CIN Ⅱ was 80.6%(54/67), and with CINⅢ was 82.4%(14/17), pathological grades and HPV E6/E7 mRNA positive rate showed a linear correlation (χ2= 26.426, P<0.01; r=0.438, P<0.01); and the number of copies correlated with the increase of pathological grades too(r=0.543, P<0.01). (3) Screening effectiveness analysis results showed, the sensitivity of HPV E6 / E7mRNA was 84.6% while TCT was 47.7%. The sensitivity and specificity were 40.0% and 91.1% respectively when HPV E6/E7 mRNA and TCT processed as sequential detection test. The CIN2 + (CIN Ⅱ and CIN Ⅲ) best diagnostic critical point of 890.26 copies/ml, was established using receiver operating characteristic (ROC) curve. The sensitivity and specificity were 58.5% and 93.7%, respectively. Conclusions The sensitivity of HPV E6/E7 mRNA test is better than TCT, the specificity is high in HPV E6/E7 mRNA and TCT processed as sequential detection test. Using the optimal cut-off value of ROC curve to detect CIN2+ has high sensitivity and specificity, so the detection of HPV E6/E7 mRNA may have some clinical value in screening and risk assessment of precancerous lesions of the uterine cervix.(Chin J Lab Med, 2015, 38: 532-536) Key words: Cervical intraepithelial neoplasia; Papillomaviridae; Oncogene proteins, viral; Papillomavirus E7 proteins

Objective To discuss the application value of DNA quantitative analysis and thinprep cytologic test for the diagnosis of malignant peritoneal effusion. Methods Fifty-eight cases patients with pleural effusion and ascites in the Second People′s Hospital of Yueyang were involved in this study.DNA quantitative analysis and thinprep cytologic test were conducted for the collected ascites, than compared the sensitivity of that two methods. Results There were 39 cases patients were diagnose malignant tumor by biopsy or pathology, the positive rate of DNA quantitative analysis(92.31%, 36/39) was obviously higher than that of thinprep cytologic test(82.05%, 32/39), the results had no statistical significance(P=0.017). The consistency check kappa value of DNA quantitative analysis and Thinprep cytologic test with pathology diagnosis were 0.807 and 0.498 respectively.The AUC of the combined examination of DNA quantitative analysis and thinprep cytologic test for malignant peritoneal effusion was 0.92, higher than the AUC of DNA quantitative analysis and Thinprep cytologic test single index examinations(0.90, 0.75). The detection sensitivity of DNA quantitative analysis and thinprep cytologic test were 94.74%(36/38) and 84.21%(32/38) respectively, specificity were 85.00%(17/20) and 65.00%(13/20) respectively, the dia were 91.38%(53/58), 77.59%(45/58) respectively, the joint detection sensitivity was 94.87%(37/39), specificity was 95.00%(19/20), the diagnostic accuracy was 93.10%(54/58). Conclusion Compared with thinprep cytologic test, DNA quantitative analysis with high sensitivity and specificity, while the combination of the two methods can greatly improve the accuracy of diagnosis of malignant ascites. Key words: Ascites; DNA quantitative analysis; Thinprep cytologic test

To estimate the status of coincidence of high-risk HPV (HR-HPV) test and thinprep cytology test(TCT) with biopsy histopathological diagnosis. And explore the diagnostic value in the cervical cancer and precancerous lesions by combination of these two methods. Retrospective analysis cases with the positive cytological diagnosis. Acrodding to the principle of voluntariness and informed consent, 3197 cases were selected and further investigated by high-risk human papillomavirus testing and biopsy histopathological diagnosis. We had a comparative analysis to the coincidence of TCT, high-risk HPV-DNA test and biopsy histopathological diagnosis. Among 3197 cases, 58.6% cases with chronic inflammation, 26.1% cases with condyloma or CIN I, 14.1% cases with CIN II-III, and 1.2% cases with invasive cervical carcinoma. Compared with pathological biopsy, the coincident rate of the diagnosis of TCT cytology and histopathology were 21.2% (ASC-US), 28.6% (ASC-H), 39.6% (LSIL), 56.2% (HSIL) and 72.4% (cervical carcinoma), respectively. Among cases of positive TCT diagnosis, Compared HR-HPV test and histopathological diagnosis, infection rate of HR-HPV increases significantly with increasing pathological grade (chi2 = 292.354, P = 0.000 < 0.05). As the TCT diagnostic level increases, the positive rate of HR-HPV marked grows (chi2 = 144.113, P = 0.000 < 0.05). TCT can reduce the incidence of cancer effectively. But lower sensitivity in the low-grade cervical lesions may cause missed diagnosis. Combined TCT and HR-HPV test will improve the detection rate of cervical lesions; it is an ideal method to screening cervical cancer.

Objective To compare the cytology diagnostic accuracy of DNA quantitative cytology and thinprep cytology test(TCT) in cervical cancer screening for exploring effective method in cervical cancer screening.Methods TCT and DNA quantitative cytology were carried out in 7 470 women.Women with positive results additionally underwent high risk human papillomavirus (HPV) detection.Positive cytologic diagnosis included atypical squamous cells(ASC) or above in TCT and DNA index 2.5 or above in DNA quantitative cytology.Results The positive rate was 13.0％ in method of DNA quantitative cytology and 13.7％ in method of TCT in 7 470 cases.Positive rate of the two methods had no significant difference in cervical cancer screening(x2 =1.813,P =0.178).There was significant difference in positive rate of TCT between cases with DNA index≥2.5,＜4.5,heteroploid cells more than 3 or DNA index≥4.5 and cases with DNA index≥2.5,＜4.5,heteroploid cells less than 3.Every grade of TCT abnormality had abnormal DNA index.Abnormality of DNA index had an increasing trend with the severity of TCT.Infection rate of high risk HPVs had significant difference in different grades of DNA index (x2 =62.648,P =0.000).Conclusion Combination of DNA quantitative cytology and TCT is an effective method in cervical cancer screening,which can reduce misdiagnosis,guide cervical biopsy and suggest infection of high risk of HPVs. Key words: DNA index; Quantitative DNA cytology; Cervical neoplasms

Objective To investigate the application value of combination of thinprep cytologic test (TCT), human papilloma virus (HPV), c-MYC and human chromosome telomerase gene (hTERC) genes to screen cervical cancer. Methods A total of 230 cases of the study objects was detected with TCT and HP, respectively. Amplification of c-MYC and hTERC genes was tested with fluorescence in situ hybridization (FISH) method. The histopathological results were the gold standard, and the sensitivity, specificity and accuracy of cervical intraepithelial neoplasia (CIN) Ⅱ/Ⅲ and squamous cell carcinoma (SCC) were compared with the four combined detection. Results Of 230 screened patients, there were 124 cases of TCT positive, 155 cases of HPV positive, c-MYC gene amplified in 118 cases, and hTERC gene amplified in 128 cases. When TCT, HPV, c-MYC, and hTERC were used alone, the highest sensitivity was HPV (84.5%), the highest specificity was c-MYC (97.6%), and the highest accuracy was hTERC (85.2%). When the three indexes were used in coordination with each other, the sensitivity and accuracy of TCT+ HPV+ hTERC were the highest (98.6% and 90.9%), and the specificity of TCT+ c-MYC+ hTERC was the highest (79.3%). When the four indexes were used together, the sensitivity was 98.6%, the specificity was 72.0%, and the accuracy was 89.1%. Conclusions Combined examination can improve the sensitivity and accuracy of screening cervical lesions, and the three combination of TCT+ HPV+ hTERC had the best effect, and c-MYC gene detection had the highest specificity. Key words: Biopsy; Papillomaviridae; Genes, myc; Telomerase; Uterine cervical neoplasms/DI

Cervical cancer is a commonly-encountered malignant tumor in women. Cervical screening is particularly important due to early symptoms being deficient in specificity. The main purpose of the study is to assess the application value of cervical thinprep cytologic test (TCT) and human papillomavirus (HPV) detection in screening for cervical cancer and precancerous lesions. In the study, cervical TCT and HPV detection were simultaneously performed on 12,500 patients selected in a gynecological clinic. Three hundred patients with positive results demonstrated by cervical TCT and/or HPV detection underwent cervical tissue biopsy under colposcopy, and pathological results were considered as the gold standard. The results revealed that 200 out of 12,500 patients were abnormal by TCT, in which 30 cases pertained to equivocal atypical squamous cells (ASCUS), 80 cases to low squamous intraepithelial lesion (LSIL), 70 cases to high squamous intraepithelial lesion (HSIL) and 20 cases to squamous cell carcinoma (SCC). With increasing pathological grade of cervical biopsy, however, TCT positive rates did not rise. Two hundred and eighty out of 12,500 patients were detected as positive for HPV infection, in which 50 cases were chronic cervicitis and squamous metaplasia, 70 cases cervical intraepithelial neoplasia (CIN) I, 60 cases CIN II, 70 cases CIN III and 30 cases invasive cervical carcinoma. Two hundred and thirty patients with high-risk HPV infection were detected. With increase in pathological grade, the positive rate of high-risk HPV also rose. The detection rates of HPV detection to CIN III and invasive cervical carcinoma as well as the total detection rate of lesions were significantly higher than that of TCT. Hence, HPV detection is a better method for screening of cervical cancer at present.

Objective To investigate the application value of human papillomavirus (HPV) combined with thinprep cytology test (TCT) detection in screening of cervical cancer. Methods 276 patients were enrolled to have HPV and TCT tests at Department of Gynecology, Shanxi Cancer Hospital from January to December in 2014. Pathological examine was applied to the patients with abnormal results of HPV and TCT. The diagnostic value of HPV, TCT and their combinations was evaluated by the diagnostic sensitivity and the rate of coincidence with the pathological examine results. Results The sensitivity of HPV and its coincidence with the pathological examine results were 81.52 % (225/276) and 79.56 % (179/225), respectively, while those of TCT were 87.68 % (242/276) and 76.86 % (186/242), respectively. The coincidence rate between the combination method and the pathological examination was higher (94.71 % , 197/208) than the individual test. Conclusion HPV combined with TCT detection can reduce the misdiagnosis rate and improve the diagnostic accuracy rate, indicating that this method is of great value in the cervical cancer screening. Key words: Human papillomavirus; Thinprep cytology test; Cervical diseases

Objective: To investigate the use of p16(INK4a) immuno-stained cytology as the primary screening for cervical cancer prevention. Methods: From March to August 2018, 902 women from Shenzhen and surrounding area were recruited for cervical cancer screening with ThinPrep Cytologic Test (TCT), cobas4800 HPV test, and p16(INK4a) co-test. Colpo/biopsies were performed using the point of interest biopsy protocol of directed and random cervical biopsies plus endocervical curettage for all women, any of whose tests was positive. Two senior cytopathologists interpreted TCT and p16(INK4a) test. The performance of p16(INK4a) for early detection of CIN2+ and inter-observer reproducibility of the interpretation of p16(INK4a) were evaluated. Results: The positive rates of HPV test, p16(INK4a) co-test and TCT diagnosed as LSIL/AGC or higher grade were 8.1% (73/902), 6.8% (61/902) and 4.7% (42/902), respectively. Colposcopy referring rate was 79.6% (109/137), among which 10 cases were diagnosed as CIN2+ (5 cases of CIN2 and 5 cases of CIN3). The sensitivity and specificity for CIN2+ of p16(INK4a) test, TCT (LSIL/AGC or higher grade) and HPV test were 90.0%, 80.0%, 100.0% and 90.9%, 91.9%, 82.5%, respectively. Compared to TCT and HPV test, there was no significant difference in sensitivity and specificity between p16(INK4a) and TCT/HPV test (P>0.05). The Kappa value of the 2 cytopathologists in interpreting p16(INK4a) and TCT was 0.944 and 0.425, respectively (P<0.05). Conclusions: p16(INK4a) for cervical cancer screening is equally sensitive to HPV test and specific to TCT while subjective difference of cytopathologists' interpretation of p16(INK4a) is small. Therefore, p16(INK4a) can be used as a new cervical cancer screen method for its better diagnostic performance.

Objective To survey the status quo of cervical cancer and cervical precancerous lesion among married women age 35 to 55 in Taishan City,Province and to investigate precautions against cervical cancer.Methods By the request of the government,free cervical cancer screening was carried out from 2010 to 2011,26 879 women in 2010 and 23 197 women in 2011 completed the questionnaire and were checked by gynecological examination and thinprep cytologic test(TCT) voluntarily,and women screened positive by TCT accepted the colposcopy-guided biopsy.Results In 2010,2491 cases of screening positive by TCT,accoanted for 9.47％ ;665 cases of cervical intraepithelial neoplasia ( CIN ),incidence rate was 2.47％,35 cases of invasive cervical cancer ( ICC ),incidence rate was 0.13 ％.In 2011,2038 cases of screening positive by TCT,accounted for 8.78 ％ ;314 cases of cervical intraepithelial neoplasia( CIN ),incidence rate was 1.35％,16 cases of ICC,incidence rate was 0.07％.Conclusion Carrying out cervical cancer screening,could detect CIN in time,give treatment of precancerous lesions as soon as possible and prevent cervical cancer. Key words: Cervix neoplasms; Data collection