Abstract
ObjectivesBlood lactate measurements are common as a marker of skeletal muscle metabolism in sport medicine. Due to the close equilibrium between the extracellular and intramyocellular space, plasma lactate is a more accurate estimate of muscle lactate. However, whole blood-based lactate measurements are more convenient in field use. The purpose of this investigation was therefore (1) to establish a plasma-converting lactate formula for field use, and (2) to validate the computed plasma lactate levels by comparison to a laboratory standard method. Design and methodsA total of 91 venous samples were taken from 6 individuals with type 1 diabetes during resting and exercise conditions and assessed for whole blood and plasma lactate using the YSI 2300 analyzer. A linear model was applied to establish a formula for converting whole blood lactate to plasma lactate. The validity of computed plasma lactate values was assessed by comparison to a laboratory standard method. ResultsWhole blood YSI lactate could be converted to plasma YSI values (slope 1.66, intercept 0.12) for samples with normal hematocrit. Computed plasma levels compared to values determined by the laboratory standard method using Passing-Bablok regression yielded a slope of 1.03 (95%CI:0.99:1.08) with an intercept of -0.11 (95%CI:-0.18:-0.06). ConclusionsWhole blood YSI lactate values can be reliably converted into plasma values which are in line with laboratory determined plasma measurements.
Highlights
Blood lactate is an important parameter in sports physiology experiments, many of which take place in the field
Computed plasma lactate values based on the conversion formula correlated well with YSI 2300 measured plasma lactate concentrations and regression analysis revealed a slope of 0.98 (95%CI1⁄40.95:1.00) and an intercept of 0.11 (95%CI1⁄40.07:0.14)
The correlation between the computed YSI plasma lactate values and the lactate measurements obtained from the laboratory method (MODULAR) is shown in Fig. 2)
Summary
Blood lactate is an important parameter in sports physiology experiments, many of which take place in the field. Plasma concentrations are considered to be a better estimate of muscle lactate due to the close equilibrium between the intramyocellular and extracellular compartment [1]. The YSI 2300 analyzer is considered to be a rapid, accurate and reliable measurement device and is commonly used for field testing of lactate in non-hemolyzed whole-blood specimens. It is well known that the extracellular fluid concentration displayed on the instrument does not represent the plasma lactate concentration. This discrepancy results from the fact that the YSI 2300 analyzer employs a volume-dependent measurement method, and the erythrocytes exert a diluting effect [4]
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