Abstract
Lipoxygenases (LOXs) are non-haem iron-containing dioxygenases and play vital roles in a variety of plant biological processes. Here, we first carried out the genome-wide identification of LOX genes in watermelon. A total of 16 LOX genes were identified, which could be classified into two categories according to phylogenetic analysis: the 9-LOXs (ClLOX1–4, 12, and 15) and 13-LOXs (ClLOX5–11, 13, 14, and 16). Furthermore, the protein structures, intrachromosomal distributions, and gene structures were thoroughly analyzed. Cis-element analysis of the promoter regions indicated that the expression of ClLOX genes may be influenced by stress and plant hormones. Bioinformatic and expression analyses revealed that the expression of ClLOX genes is tissue-specific and hormone-responsive. The detected LOX genes exhibited distinctive expression patterns in various tissues. Different ClLOX genes showed different responses to methyl jasmonate (MeJA), salicylic acid (SA), and ethylene (ET) treatments, particularly ClLOX7, which exhibited the most active response to the above treatments. This study provides valuable information for a better understanding of the functions of LOX genes and further exploration of the LOX gene family in watermelon.
Highlights
Lipoxygenases (LOXs; EC 1.13.11.12) belong to a family of non-haem iron-containing dioxygenases that are widely present in plants, animals, fungi, and even in bacteria [1,2,3,4]
The full-length LOX protein sequences in Arabidopsis and rice were downloaded according to a previous study [17], and used as queries to search against the watermelon (Citrullus lanatus subsp. vulgaris cv. 97103) v1 proteome with the BLASTP
By HMMER search and BLASTP, a total of 19 LOX genes were identified in watermelon (C. lanatus) genome, three of which were removed for shortness and not having the complete domain of LOX as tested by SMART and Pfam
Summary
Lipoxygenases (LOXs; EC 1.13.11.12) belong to a family of non-haem iron-containing dioxygenases that are widely present in plants, animals, fungi, and even in bacteria [1,2,3,4]. They can catalyze the oxidation of polyunsaturated fatty acids (PUFAs), such as linoleic acid (LA) and α-linolenic acid (α-LeA), into unsaturated fatty acid hydroperoxides [2,5]. Plant LOXs have a highly conserved lipoxygenase domain at the C-terminus and a PLAT/LH2. Based on their primary structure and overall sequence similarity, plant LOXs can be classified
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