Abstract
The stoichiometry of free NADPH oxidation in phenobarbital induced rabbit liver microsomes was measured by means of registering the rates of NADPH, H + and O 2 consumption and O 2 • and H 2O 2 production. ΔO 2 • :ΔH 2O 2 ratio is approximately I indicating that about half H 2O 2 results from O 2 • dismutation, the second half being formed directly. ΔNADPH:ΔH 2O 2 and ΔO 2:ΔH 2O 2 ratios exceed I and therefore another product of the reaction is water. The fact that the ratio (ΔNADPH-ΔH 2O 2):(ΔO 2-ΔH 2O 2) is 2 allows one to consider direct 4-electron O 2 reduction as the major way of water formation rather than endogenous substrate hydroxylation.
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