Abstract
This is a report of the complete genome sequences of plaque-selected isolates of each of the five virus strains included in a South African commercial trivalent bluetongue virus (BTV) attenuated live virus vaccine, a BTV-4 field strain isolated from Rustenburg, South Africa, in 2011, and a bluetongue reassortant (bluetongue virus 4 strain 4/O. aries-tc/ZAF/11/OBP-115) isolated from experimentally vaccinated cattle. Full-genome sequencing and phylogenetic analyses show that the bluetongue virus 9 strain 9/B. taurus-tc/ZAF/15/Onderstepoort_B02b is a reassortant virus containing segments from both BTV-9 and BTV-8.
Highlights
Bluetongue virus (BTV) is a noncontagious disease of ruminants that is transmitted by Culicoides biting midges (Diptera: Ceratopogonidae) [1]
In South Africa, a polyvalent BTV-modified live virus (MLV) vaccine is manufactured by Onderstepoort Biological Products (OBP) Ltd
We report the fullgenome sequences of BTV-3, BTV-8, BTV-9, BTV-10, and BTV11, which were isolated from bottle B of the BTV-MLV vaccine, a BTV-4 field strain isolated from a clinically infected ewe in Rustenburg, and a reassortant strain between BTV-9 and BTV-8 segment 8 isolated from experimentally vaccinated cattle
Summary
Bluetongue virus (BTV) is a noncontagious disease of ruminants that is transmitted by Culicoides biting midges (Diptera: Ceratopogonidae) [1] (genus Orbivirus, family Reoviridae). The genome of BTV is composed of 10 segments of double-stranded RNA (dsRNA) that collectively encode seven structural proteins and five nonstructural proteins [2,3,4].
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