Abstract
The chrysanthemum stunt viroid (CSVd), a member of the genus Pospiviroid with a single circular RNA genome, infects many chrysanthemum species. Here, we report 25 complete genome sequences of CSVd in a single chrysanthemum cultivar, revealing 20 variants.
Highlights
The chrysanthemum stunt viroid (CSVd), a member of the genus Pospiviroid with a single circular RNA genome, infects many chrysanthemum species
To obtain information about quasi-species in a single chrysanthemum plant, we screened several commercial chrysanthemum cultivars for CSVd infection by reverse transcription (RT)-PCR using CSVd-specific primers (5=-AAAGAA ATGAGGCGAAGAAGTC-3= and 5=-TTCTTTCAAAGCAGCAGG GT-3=)
To obtain information of CSVd quasi-species in the Disk club cultivar, we conducted RT-PCR to amplify the complete genome of CSVd
Summary
The chrysanthemum stunt viroid (CSVd), a member of the genus Pospiviroid with a single circular RNA genome, infects many chrysanthemum species. Viroids are the smallest pathogens, consisting of a single circular RNA genome, which does not encode any protein [1, 2]. The chrysanthemum stunt viroid (CSVd) is a member of the genus Pospiviroid in the family Pospiviroidae [3, 4]. A wide range of chrysanthemum species are major hosts for CSVd, but CSVd infects other plant species, including Ageratum, Dahlia, Petunia hybrida, and Senecio [5].
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