Abstract
Here, we report on the closed genome sequence of Clostridium pasteurianum DSM 525, which is an anaerobic, Gram-positive and endospore-forming organism. C. pasteurianum can fix N2 and produce solvents such as butanol and 1,3-propanediol from carbohydrates. The genome consists of a single 4,350,673-bp replicon.
Highlights
We report on the closed genome sequence of Clostridium pasteurianum DSM 525, which is an anaerobic, Gram-positive and endospore-forming organism
In contrast to most other solventogenic clostridia, C. pasteurianum is able to grow with glycerol as sole carbon and energy source [1, 2] and couple glycerol breakdown with a highly active butanol-producing pathway The major products during glycerol degradation are 1,3-propanediol, ethanol, and butanol [1, 3]
The complete genome of C. pasteurianum DSM 525 consists of a single chromosome of 4,350,673 bp with an overall GϩC content of 30%
Summary
We report on the closed genome sequence of Clostridium pasteurianum DSM 525, which is an anaerobic, Gram-positive and endospore-forming organism. The Gram-positive anaerobic spore-forming bacterium Clostridium pasteurianum DSM 525, is able to produce butanol from carbohydrates [1]. In contrast to most other solventogenic clostridia, C. pasteurianum is able to grow with glycerol as sole carbon and energy source [1, 2] and couple glycerol breakdown with a highly active butanol-producing pathway The major products during glycerol degradation are 1,3-propanediol, ethanol, and butanol [1, 3]. Chromosomal DNA of C. pasteurianum DSM 525 was isolated using the MasterPure complete DNA purification kit (Epicentre, Madison, WI, USA).
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