Abstract
Stenotrophomonas maltophilia is a Gram-negative bacterium associated with multidrug-resistant nosocomial infections, a problem for immunocompromised patients and those with cystic fibrosis. Here, we present the new S. maltophilia-infecting podophage Pokken. Its 76,239-bp genome, with 92 protein-coding genes and 5 tRNA genes predicted, is similar to that of phage N4.
Highlights
Stenotrophomonas maltophilia is an emerging Gram-negative opportunistic pathogen [1]
Samples were negatively stained with 2% uranyl acetate and viewed by transmission electron microscopy at the Texas A&M Microscopy and Imaging Center [5]
All tools were accessed at the Center for Phage Technology Galaxy interface, and Web Apollo was used for annotation [18, 19]
Summary
Stenotrophomonas maltophilia is an emerging Gram-negative opportunistic pathogen [1]. Pokken was isolated from filtered (filter size, 0.2 m) freshwater collected at Camp Samples were negatively stained with 2% (wt/vol) uranyl acetate and viewed by transmission electron microscopy at the Texas A&M Microscopy and Imaging Center [5]. DNA was purified with the modified Promega Wizard DNA clean-up system shotgun library preparation protocol, prepared as Illumina TruSeq libraries with a Nano low-throughput kit, and sequenced on an Illumina MiSeq instrument with paired-end 250-bp reads using v2 500-cycle chemistry [6].
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