Abstract
In this study, we present the complete genome of a new isolate of soybean dwarf virus (SbDV) (genus Luteovirus, family Luteoviridae) from white clover in Germany. The complete genome of the isolate (JKI ID 23556) consists of 5,858 nucleotides and displays 94.98% nucleotide identity to its most similar SbDV relative (GenBank accession number MN412736).
Highlights
L., family Fabaceae) is wide range of climatic one of the regions in most important the world (1)
Total RNA was extracted from infected V. faba leaves using the innuPREP RNA minikit (Analytik Jena AG), and rRNA was depleted with the RiboMinus plant kit (Invitrogen) following the manufacturers’ instructions. cDNA
A direct BLASTn search of the NCBI database showed that JKI ID
Summary
L., family Fabaceae) is wide range of climatic one of the regions in most important the world (1). In Germany, white clover is known to be infected by SbDV (2). To sequence the whole genome of SbDV JKI ID 23556, we followed the steps described previously (4). Total RNA was extracted from infected V. faba leaves using the innuPREP RNA minikit (Analytik Jena AG), and rRNA was depleted with the RiboMinus plant kit (Invitrogen) following the manufacturers’ instructions.
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