Abstract
Illumina sequencing was employed to determine the complete chloroplast (cp) genome sequence of Sphaeropteris brunoniana (S. brunoniana), which is a relict fern. The cp genome of S. brunoniana is indeed a circular DNA molecule with 156,659 bp. It includes an inverted repeats (IRs) pair with 24,011 bp each and two single-copy regions with 86,196 bp and 22,441 bp, respectively. Additionally, the genome contains 117 unique genes encoding 85 proteins, 28 tRNAs, four rRNAs. Pseudogenes of ycf66 and trnT-UGU are also detected in this genome.Bayesian phylogenetic tree strongly supports the deduction that S. brunoniana belongs to Cyatheaceae. To date, this is the first cp genome for the genus S. brunoniana.
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