Abstract
BackgroundThe nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content.ResultsA soybean near-isogenic line (NIL) pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than 8.4 Mbp of genomic sequence on chromosome 20. Using Affymetrix® Soy GeneChip and high-throughput Illumina® whole transcriptome sequencing platforms, 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the 8.4 Mbp LG I protein QTL region.ConclusionsThis study identifies gene candidates at the LG I protein QTL for potential involvement in the regulation of protein content in the soybean seed. The results demonstrate the power of complementary approaches to characterize contrasting NILs and provide genome-wide transcriptome insight towards understanding seed biology and the soybean genome.
Highlights
The nutritional and economic value of many crops is effectively a function of seed protein and oil content
near-isogenic line (NIL) populations used to map the Linkage Group I (LG I) protein quantitative trait locus (QTL) were created by backcrossing the high protein G. soja (PI468916) allele into a G. max background (A81-356022) [32]
We found no significant differences in transcript expression of acetyl-CA carboxylase (ACCase) and phosphoenolpyruvate carboxylase (PEPC) between NILs, we observed that transcripts corresponding to several forms of ACCase and PEPC were expressed at all stages of seed development in this study
Summary
The nutritional and economic value of many crops is effectively a function of seed protein and oil content. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content. Seed protein and oil are crucial to the value of many crop species. Soybean (Glycine max (L.) Merrill), protein and oil are primary nutritional components of mature seed. Protein and oil comprise some 40% and 20%, respectively, of soybean seed. A better understanding of the genetic basis of seed protein variation is important for developing strategies to improve seed quality traits in soybean and in other legumes and cereal grains
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