Abstract
Sheep antibody-sensitized human erythrocytes (SAHE) were haemolyzed by sheep, goat, cow, pig, guinea-pig (g-p), rat, hamster, horse, cat, dog, chicken, mouse, rabbit and human serum. Mg 2+-ethyleneglycoltetraacetic acid (EGTA) plasma from these species, titrated in the presence of Mg 2+-EGTA, were also haemolytic. Preserved g-p serum was non-haemolytic for SAHE. All serum samples haemolyzed rabbit antibody-sensitized sheep erythrocytes (RASE) but most of the plasma samples did not. Serum and plasma from many species haemolyzed human and sheep erythrocytes per se. Except for fresh g-p plasma, all plasmas heated at 50°C for 30 min failed to haemolyze SAHE. A CM-cellulose fraction of foetal lamb serum restored haemolytic activity to sheep, goat, cow, pig, horse, cat, dog and chicken plasma in the presence of Mg 2+-EGTA. These results indicate that an alternative pathway-like mechanism appears to be involved in haemolysis of SAHE by plasma in the presence of Mg 2+-EGTA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
