Comparison on model establishment methods and index in common of Yang deficiency mice induced by hydrocortisone

Abstract

Objective To compare and analyze the index variation of Yang deficiency mice induced by hydrocortisone in different way of administration and dose. Methods The mice model of Yang deficiency were induced by low dose and high dose hydrocortisone（12.5 mg/kg, 25 mg/kg） in two ways of intramuscular and intraperitoneal injection. The symptoms, body weight, the average intake, the index of stress and the coefficient of sex organs and immune organs of animals were observed. Results In the way of intraperitoneal injection, the weight（24.52±3.29）g, body temperature（35.24±0.32）℃ of high dose of model group were significantly lower than that of control group（31.10±6.11）g,（37.02±0.64）℃. The average intake of low dose group（4.30 ± 0.29）g/（per？d） was lower than control group（7.38±0.53）g/（per？d）, and the coefficient of preputial glands（0.10±0.02）, penis（0.15±0.03）, thymus（0.12±0.03）were lower than that of control group. In the way of intramuscular injection, the average intake of high dose group（5.92±2.01）g/（per？d） was lower than control group（8.60±1.33）g/（per？d）. The body temperature（34.90±0.22）℃ and the time of swimming in low temperature （34.00±22.41）s of low dose model group were lower than that of control group（36.43±0.91）℃, （67.17±21.93）s, and the coefficient of thymus of two model groups（0.10±0.02）,（0.11±0.06）were lower than that of control group. Conclusion Various dose and model establishment methods of Yang deficiency mice have different the time of symptom appearing, the degree of symptom and sensitive index. Key words: Hydrocortisone; Mice; Yang deficiency; Model; Index