Abstract
Two culture media were compared for their ability to isolate Clostridium difficile from environmental sites within a UK hospital. The media were cefoxitin-cycloserine-egg yolk agar plus lysozyme (CCEY/L) and chromID C.difficile. A wide range of environmental surfaces was sampled using sterile sponges (Polywipes) and these were inoculated on to both media. C.difficile was recovered from 105 of 496 sites (21%) using a combination of both media. The sensitivity of chromID C.difficile was 87.6% compared with 26.6% for CCEY/L (P<0.0001). chromID C.difficile performed significantly better than CCEY/L for the recovery of C.difficile from the environment.
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