Abstract

Background: The Kato-Katz technique is recommended for diagnosis of Schistosoma mansoni infection by the World Health Organization. However, egg counts are subject to variability. The aim of this study is to determine the prevalence of S. mansoni infection in school children using two different techniques and to recommend the technique that should be routinely used in the diagnosis of this infection. Methodology: Field investigations on faecal samples from 299 Cameroonian school children were carried out in 2016 to compare the effectiveness of the Kato-Katz and Formalin-ether techniques in diagnosis of S. mansoni infections. Results: Schistosome eggs were detected in 37 (12.3%) samples with the Kato-Katz technique and 61 (20.4%) samples with the Formalin-ether technique. The difference between the prevalence observed for the two techniques was significant in males and age group 10 - 12 years ( p < 0.5). Conclusion: The Formalin-ether technique was more sensitive than the Kato-Katz method for detecting S. mansoni eggs in faecal matter. Despite its cost, the Formalin-ether technique can be routinely used in the laboratory for epidemiological studies of intestinal schistosomiasis Keywords: Formalin-ether, Kato-Katz Schistosoma mansoni , school children

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