Comparison of the Growth and Survival of Larval Turbot in the Absence of Culturable Bacteria with Those in the Presence of Vibrio anguillarum, Vibrio alginolyticus, or a Marine Aeromonas sp

Abstract

Larval turbot (Scophthalmus maximus) were reared on rotifers (Brachionus plicatilis) in the absence of culturable bacteria for up to 14 days and exhibited growth and high rates of survival (>55% in five experiments). Low numbers of known bacteria were introduced into similar cultures by exposure of the rotifers to a suspension of bacteria prior to addition of rotifers to the larval cultures; Vibrio anguillarum 91079 caused a highly significant decrease (P <0.01) in the proportion of survivors in two separate trials. With an Aeromonas sp. previously isolated from a healthy batch of copepod-fed larvae, there was no significant difference in survival compared with control larvae, even though the density of bacteria in the water of larval cultures reached 10(sup7) ml(sup-1). Bacteria colonized the gut of larvae exposed to Aeromonas-treated rotifers to levels similar to those in conventionally reared fish (>4 x 10(sup4) CFU per larva). Rearing of larvae in the presence of known bacteria provides a means of investigating the interaction of specific bacteria with turbot larvae and could provide a method for the selection of bacteria which may restrict the growth of opportunistic pathogens which would be harmful to turbot larvae.