Abstract
Campylobacter is one of the most common pathogens leading to the bacterial diarrheal illness. In order to set up one effective culture independent assay for the screen of the Campylobacter infection in the diarrheal patients, the quadruple real-time PCR method comparing to the culture based on the enriched filtration method which was recognized as the most effective isolation method was assessed for 190 stool samples from the diarrheal patients collected during the Foodborne Diseases Active Surveillance Network in Beijing. This multiple real-time PCR was designed to identify the Campylobacter genus, C. jejuni, C. coli, and C. lari simultaneously. With the enrichment culture method, 23 (12.1%, 23/190) Campylobacter isolates were obtained (20 C. jejuni and 3 C. coli), however, 31 samples (16.3%, 31/190) were detected positively with the real-time PCR (21 C. jejuni, 8 C. coli, and 2 Campylobacter genus only). With the comparison, the real-time-PCR method is more sensitive than the enrichment filtration method (16.3 vs. 12.1%, p = 0.021). Among the culture-positive samples, 95.7% (22/23) were detected positively by PCR which indicate the specificity of this method was higher. These two methods were consistent well (Kappa = 0.785, p < 0.05). Comparing to the culture methods, the result of the multiple real-time PCR method is sensitive, reliable and rapid. The present study indicated this multiple real-time PCR can be used both for the surveillance network and the preceding screen for bacteria isolation. This is first comparative study between the culture and multiple real-time PCR method for Campylobacter identification in stool specimens from the diarrheal patients.
Highlights
Foodborne Campylobacter is recognized as the leading causes of the bacterial diarrheal illness in both developing and developed countries (Coker et al, 2002; EFSA, 2015; Marder et al, 2017; Li et al, 2018; Natsos et al, 2018)
The incidence of the culture confirmed and Cultureindependent diagnostic tests (CIDTs) positive infections was highest for Campylobacter among bacterial and parasitic infections per 100,000 persons in United States (Marder et al, 2017)
A total of 190 stool samples from diarrhea patients were collected in Shunyi and Konggang Hospitals in Shunyi district, Beijing from July 2017 to February 2018. 190 specimens were collected from 190 cases
Summary
Foodborne Campylobacter is recognized as the leading causes of the bacterial diarrheal illness in both developing and developed countries (Coker et al, 2002; EFSA, 2015; Marder et al, 2017; Li et al, 2018; Natsos et al, 2018). Cultureindependent diagnostic tests (CIDTs) are increasingly used to diagnose Campylobacter infection in the Foodborne Diseases Active Surveillance Network (FoodNet) in United States and other countries (Bessède et al, 2011). The incidence of the culture confirmed and CIDT positive infections was highest for Campylobacter among bacterial and parasitic infections (confirmed = 11.79; CIDT positive–only = 17.43) per 100,000 persons in United States (Marder et al, 2017)
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