Abstract

Abstract In this study the influence of collection media and sampling method on endotoxin extraction for a variety of endotoxin-contaminated aerosols was examined. A first set of experiments compared endotoxin concentrations on eight different filter media after sampling from a chamber containing a saline endotoxin aerosol. Glass fiber (GF) and cellulose acetate (CA) filters were equivalent and showed significantly higher amounts of extractable endotoxin than any other filter type. Polysulfone, polyvinyl chloride (PVC), and polytetrafluoroethylene (PTFE) filters indicated two-thirds of the endotoxin concentration of GF and CA filters; concentrations on nylon, polycarbonate, and silver membrane filters were significantly lower (one-tenth that of GF and CA filters). The presence of chloride anion was comparable for all filter media from which significant amounts of endotoxin could be extracted, suggesting that extraction of endotoxin, but not chloride anion, was dependent on filter type. In subsequent exper...

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