Abstract
In a recent paper in this journal it was shown that human ceruloplasmin contains a single continuous polypeptide chain composed of about 1050 amino acid residues. Together with the carbohydrate this accounts for the molecular weight of the native protein, 132000. In contrast to this porcine ceruloplasmin has been reported to contain two subunits of molecular weight 70000–80000. This was the reason for performing a direct comparison of polypeptide‐chains from ceruloplasmin from different mammalian sera.Ceruloplasmin was prepared from sera of man, pig, horse and rabbit. The reduced and alkylated proteins were gel filtered on a calibrated column of 6% agarose in 6 M guanidine hydrochloride. The four proteins all contained a single main component eluting in the same position within errors of measurements. It is concluded that the four proteins investigated all contain a single polypeptide chain of very similar size.
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