Abstract
Mycoplasma pneumoniae is a common etiologic agent of community-acquired respiratory disease whose rapid laboratory diagnosis is complicated. We developed a culture-amplified immunological antigen detection assay which employs a monoclonal antibody for the recognition of a polypeptide antigen of 43kDa in an immunoblotting technique. Polyclonal antisera to the same antigen were obtained from rabbit and hamster immunization with affinity chromatography-purified antigen. The latter sera, sera from natural human infection and experimental hamster infection, and a pool of anti-43kDa monoclonal antibodies were compared to the single anti-43kDa monoclonal antibody, OC2F5. Among these, only polyclonal hamster anti-43kDa sera were able to enhance antigen detection quantitatively. This enhancement however did not result in a significant benefit to the culture-amplified system.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
