Abstract
Volatile and non-volatile organic acids were analysed by high-performance liquid chromatography (HPLC) on an Aminex HPX-87H, gas-solid chromatography (GSC) on a Porapak Q and gas-liquid chromatography (GLC) on a fused-silica capillary column. The results were compared by using standard acid solutions and with culture media of two strains of alkaliphilic Bacillus. Whereas the resolution of acids was excellent with GLC, the quantitative reproducibility was better with HPLC. Identification of complex culture mixtures was accomplished by GC-MS. Applicability of the methods for different purposes is discussed.
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