Abstract
Comparison of five viral nucleic acid extraction kits for the efficient extraction of viral DNA and RNA from cell-free samples
Highlights
The ability to extract high purity viral nucleic acid, either DNA or RNA, is required for many downstream molecular and medical techniques used in research or diagnostic purposes
The procedure determines the copy number, commonly known as the “viral load”, in the blood of patients infected with certain virtues, such as hepatitis B, hepatitis C, human immunodeficiency virus (HIV), and many other viral infections [4,5,6]
It is expected that the concentration of viral DNA or RNA in patient samples are hardly detectable by using spectrophotometry
Summary
The ability to extract high purity viral nucleic acid, either DNA or RNA, is required for many downstream molecular and medical techniques used in research or diagnostic purposes. The amplification of viral nucleic acid; either DNA using qualitative real-time PCR (qPCR), or RNA using qualitative revers-transcription real-time PCR (qRT-PCR), is widely used in the field of molecular diagnostics [3]. Many manufacturers of real-time thermal cyclers claim that their instruments are able to detect a single nucleic acid target in samples with a slow as few copy numbers [7]; their claim is usually affected by various factors and conditions. One of the most important factors affecting the instrument’s ability to detect its nucleic acid target is the integrity of the extracted viral nucleic acid [8]
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