Comparison of endoglucanase-1 (EG1) induction in the edible straw mushroom Volvariella volvacea by lactose and/or cellobiose with or without added sorbose

Abstract

We have compared the induction of an endoglucanase (EG1) by α-lactose and/or cellobiose, with or without added L-sorbose, in submerged cultures of Volvariella volvacea, to better understand the mechanism whereby cellulase formation is triggered by these soluble disaccharides. EG1 levels induced by α-lactose and cellobiose were 28.6% and 6.7%, respectively of the highest levels recorded with crystalline cellulose. Sorbose did not induce EG1 and strongly repressed enzyme levels when added to α-lactose but not cellobiose-containing cultures. EG1 levels in cultures containing all three saccharides were similar to those recorded with sorbose and cellobiose although enzyme induction was delayed by 12 h. When V. volvacea was pre-grown for 24 h in medium containing sorbose as the sole carbon source, followed by addition of α-lactose or cellobiose or a mixture of the two, EG1 levels recorded in the α-lactose-supplemented cultures were again markedly lower compared with cultures containing only α-lactose. Maximal enzyme levels in cultures with added cellobiose or cellobiose and α-lactose were not affected although appearance of EG1 in culture supernatants was again delayed by 12 h. Semi-quantitative RT-PCR revealed that higher, more prolonged, levels of eg1 transcription occurred in V. volvacea cultures induced with α-lactose compared with cellobiose- or α-lactose + cellobiose-induced cultures. However, eg1 transcription levels in cultures induced with cellobiose or with cellobiose + lactose, and the corresponding cultures with added sorbose, were not markedly different.