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Abstract
An infrared spectroscopy-based multicomponent assay of biofluids such as whole blood, urine, and synovial fluid holds considerable promise for the clinical laboratory. The methodology is reagentless, fast and readily automated. Infrared spectroscopic analysis is not hindered by the physical state of the sample and measurements can be made in a variety of instrument configurations. In this review we present the spectra of physiological samples measured as liquids and dried films using transmission-, attenuated total reflection-, photoacoustic-and diffuse reflectance infrared spectroscopy. The advantages and limitations of each of these techniques are discussed in the context of developing a routine clinical analysis method for biofluid samples.
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