Abstract
Aim: The aim of this study is to compare between ethanol and ethyl acetate rhizome extract of K.rotunda against HeLa cervical cancer cell in vitro. Material and Methods: Methods used in this research are test the chemical compound of extracts using Thin Layer Chromatography (TLC) and phytochemical screening test, also cytotoxicity test using MTT assay. Result: Ethyl acetate extract contains flavonoid, alkaloid, tannin, and triterpenoid, while ethanol extract have flavonoid, triterpenoid, and alkaloid. In addition, ethanol extract has strong cytotoxic activity (IC50 = 16,939 μg/ml) while ethyl acetate extract has moderate cytotoxic activity (IC50 = 127,9 μg/ml). Each of extracts showed significant results (p ≤ 0,05) although when compared between concentrations there are several concentrations that are not significant and also small coefficient of determinant values caused by various confounding factors. Conclusion: The ethanol extract of K.rotunda rhizome extract has the higher cytotoxicity activity compared to ethyl acetate extract of K.rotunda rhizome extract against HeLa cervical cancer cell.
Highlights
Cervical cancer is the fifth highest cancer in the world
Thin Layer Chromatography (TLC) dan phytochemical screening are used for analyze compounds contained in both extracts while MTT Assay is used for measure the cytotoxic activity
The results of TLC are stated in Retention Factor (Rf)
Summary
Cervical cancer is the fifth highest cancer in the world. This cancer is the most common cancer in developing countries and one of the top ten cancers in developed countries. There are several studies using plant extract in order to find the alternative treatment of cervical cancer, one of which is Kaempferia rotunda rhizome extract. There are 2 types of turmeric plants, namely yellow turmeric (Curcuma domestica) and white turmeric (Kaempferia rotunda). White turmeric in Chinese pharmacology is said to have properties that can stop bleeding and increase appetite. This plant is antineoplastic (damages the formation of ribosomes in cancer cells or inhibits the growth of cancer cells). The parts used are rhizomes and leaves.[4]
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