Abstract
Variations in the conjugase treatment step of the microbiological assay for food folacin were investigated. The source of the conjugase–fresh hog kidney, fresh chicken pancreas or commercial dried chicken pancreas–did not significantly affect the measurable folacin activity of spinach. However, the buffer, pH, incubation time, sequence of steps in extraction and conjugase treatment, and method of sample filtration (filter paper vs centrifuge) used prior to conjugase treatment were all found to significantly affect the folacin activity of spinach.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
