Comparison of antioxidative and antifibrotic effects of Allium sativum in rat model of chronic pancreatitis (840.4)

Abstract

OBJECTIVE: Allium sativum (Garlic) is considered effective antioxidant. This study aimed to compare the antioxidative and antifibrotic effects of Allium sativum in L‐Arginine induced chronic pancreatitis in rats METHODS: Oral administration of freshly homogenized Allium sativum (0.25g/kg per day) started two day before induction of chronic pancreatitis. L‐arginine hydrochloride (250mg/100g bw/day) was administered intraperitoneally (IP) in 2 repeated doses of 1 hour interval on day 1, repeated with 250mg/100 g bw/day, on day 4, 7, 10, 13, 16 & 19 was used to induce CP. Histological examination by masson’s trichrome staining and measurement of reduced glutathione (GSH) and lipid peroxidation (LPO) of pancreatic tissue were performed to evaluate pancreatic damage and fibrosis. IHC staining of α‐SMA was done to evaluate the activated pancreatic stellate cells and mRNA levels (densitometry analysis) of transforming growth factor‐β1 (TGF‐β1), fibronectin 1 and collagen‐α1(I) were performed by reverse transcription polymerase chain reaction.RESULTS: Allium sativum, supplementation increased the level of GSH and decreased the level of LPO which indicates reduction in oxidative stress. It also reduces inflammation and fibrosis. It also down‐regulates the mRNA expression of TGF‐β1, fibronectin 1 and collagen‐α1(I) in L‐arginine induced CP.CONCLUSION: Oral administration of Allium sativum improves pancreatic inflammation and fibrosis in L‐arginine induced CP rats. Therefore, Allium sativum can be a novel option for alleviating inflammation and fibrotic process in CP.