Comparison between regional micropush-out and microtensile bond strength of resin composite to dentin

Abstract

Objective. In this study we compared the micropush-out (µ-PO) and microtensile (µ-TBS) test methods for resin composites on different levels and surfaces of dentin. Material and Methods. Thirty-four sound human molars were divided into two groups in accordance with the dentin surface used (occlusal (O) or mesio-distal (M)), then subdivided into a further two groups in accordance with the test method (µ-PO (P) or µ-TBS (T)). In groups OP and OT, teeth were ground occlusally perpendicular to their axis, and in groups MP and MT, mesio-distally parallel to their axis to expose dentin. Dentin disks were prepared from different regions of the teeth either in an occluso-cervical (group OP) (n=5) or mesio-distal direction (group MP) (n=5) (S, superficial; M, middle; D, deep). One-hundred-and-twenty standardized occlusal cavities were prepared in these dentin disks using a conical-shaped diamond rotary cutting instrument. The adhesive (Adper Scotchbond Multi-Purpose Dental Adhesive) and a composite material (Filtek Z250 Universal Restorative) were applied and polymerized with a LED light curing unit (Elipar FreeLight 2). The disks (n=20/per group) were tested in a universal testing machine and pushed out with a cross-head speed of 1.0 mm/min. In groups OT (n=12) and MT (n=12), the teeth were ground to expose superficial, middle, and deep dentin. Build-ups of resin composite were constructed with the same materials. The specimens were serially sectioned and trimmed to hour-glass shapes, then tested with the µ-TBS tester at a rate of 1 mm/min. Failure modes were examined using a stereomicroscope and scanning electron microscope. Results. ANOVA revealed significant differences among the groups (p<0.001). With the push-out test, no premature failure occurred, the variability of the data distribution was acceptable, and regional differences in bond strength among dentin levels could be assessed. Conclusions. The µ-PO test method could be an alternative to the µ-TBS technique.